Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2003-12-31
pubmed:abstractText
The earliest embryonic mitoses in Drosophila, as in other animals except mammals, are viewed as synchronous and of equal duration. However, we observed that total cell-cycle length steadily increases after cycle 7, solely owing to the extension of interphase. Between cycle 7 and cycle 10, this extension is DNA-replication checkpoint independent, but correlates with the onset of Cyclin B oscillation. In addition, nuclei in the middle of embryos have longer metaphase and shorter anaphase than nuclei at the two polar regions. Interestingly, sister chromatids move faster in anaphase in the middle than the posterior region. These regional differences correlate with local differences in Cyclin B concentration. After cycle 10, interphase and total cycle duration of nuclei in the middle of the embryo are longer than at the poles. Because interphase also extends in checkpoint mutant (grapes) embryo after cycle 10, although less dramatic than wild-type embryos, interphase extension after cycle 10 is probably controlled by both Cyclin B limitation and the DNA-replication checkpoint.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0950-1991
pubmed:author
pubmed:issnType
Print
pubmed:volume
131
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
401-11
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:14681192-Animals, pubmed-meshheading:14681192-Animals, Genetically Modified, pubmed-meshheading:14681192-CDC2 Protein Kinase, pubmed-meshheading:14681192-Cell Cycle, pubmed-meshheading:14681192-Chromatids, pubmed-meshheading:14681192-Cyclin B, pubmed-meshheading:14681192-DNA Replication, pubmed-meshheading:14681192-Drosophila Proteins, pubmed-meshheading:14681192-Drosophila melanogaster, pubmed-meshheading:14681192-Gene Dosage, pubmed-meshheading:14681192-Genes, Insect, pubmed-meshheading:14681192-Green Fluorescent Proteins, pubmed-meshheading:14681192-Histones, pubmed-meshheading:14681192-Luminescent Proteins, pubmed-meshheading:14681192-Microscopy, Confocal, pubmed-meshheading:14681192-Mitosis, pubmed-meshheading:14681192-Mutation, pubmed-meshheading:14681192-Recombinant Fusion Proteins, pubmed-meshheading:14681192-Time Factors
pubmed:year
2004
pubmed:articleTitle
Both cyclin B levels and DNA-replication checkpoint control the early embryonic mitoses in Drosophila.
pubmed:affiliation
Department of Biology, University of Washington, Seattle, WA 98195-1800, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.