| pubmed-article:14646616 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:14646616 | lifeskim:mentions | umls-concept:C1513095 | lld:lifeskim |
| pubmed-article:14646616 | lifeskim:mentions | umls-concept:C0003968 | lld:lifeskim |
| pubmed-article:14646616 | lifeskim:mentions | umls-concept:C0752312 | lld:lifeskim |
| pubmed-article:14646616 | lifeskim:mentions | umls-concept:C1710082 | lld:lifeskim |
| pubmed-article:14646616 | lifeskim:mentions | umls-concept:C1819445 | lld:lifeskim |
| pubmed-article:14646616 | lifeskim:mentions | umls-concept:C0591833 | lld:lifeskim |
| pubmed-article:14646616 | lifeskim:mentions | umls-concept:C0920643 | lld:lifeskim |
| pubmed-article:14646616 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:14646616 | pubmed:dateCreated | 2003-12-3 | lld:pubmed |
| pubmed-article:14646616 | pubmed:abstractText | We recently reported that interleukin-18 (IL-18) is highly expressed in malignant skin tumours such as melanomas, and may play a key role in the malignancy of such tumours. This study was designed to investigate the mechanisms of IL-18 regulation by vitamin C in B16F10 murine melanoma cells. Cells were treated with vitamin C, and the expression of IL-18 was measured by reverse transcription-polymerase chain reaction and intracellular flow cytometry analysis. Decreased IL-18 production and a significant reduction in IL-18 mRNA transcript were detected in cells treated with vitamin C. The effect of vitamin C treatment was blocked by the antioxidant N-acetyl-L-cysteine, suggesting that vitamin C affects IL-18 expression by up-regulating intracellular reactive oxygen intermediate (ROI) levels. To investigate whether the mitogen-activated protein kinase (MAPK) signalling pathway is involved in the downregulation of IL-18 production, cells were pretreated with SB203580, an inhibitor of p38 MAPK, prior to the addition of vitamin C. This pretreatment blocked the decrease in IL-18 production. However, vitamin C treatment enhanced the expression of phosphorylated p38 MAPK. Taken together, we conclude that vitamin C increases intracellular ROI levels, and regulates IL-18 production through the MAPK signalling pathway. | lld:pubmed |
| pubmed-article:14646616 | pubmed:language | eng | lld:pubmed |
| pubmed-article:14646616 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14646616 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:14646616 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14646616 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14646616 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14646616 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14646616 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14646616 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14646616 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14646616 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14646616 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14646616 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14646616 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14646616 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:14646616 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:14646616 | pubmed:issn | 0960-8931 | lld:pubmed |
| pubmed-article:14646616 | pubmed:author | pubmed-author:KimSeonghanS | lld:pubmed |
| pubmed-article:14646616 | pubmed:author | pubmed-author:LeeWang JaeWJ | lld:pubmed |
| pubmed-article:14646616 | pubmed:author | pubmed-author:ChoDaehoD | lld:pubmed |
| pubmed-article:14646616 | pubmed:author | pubmed-author:KimYoung-InYI | lld:pubmed |
| pubmed-article:14646616 | pubmed:author | pubmed-author:KimDaejinD | lld:pubmed |
| pubmed-article:14646616 | pubmed:author | pubmed-author:KimYeong... | lld:pubmed |
| pubmed-article:14646616 | pubmed:author | pubmed-author:KangJae... | lld:pubmed |
| pubmed-article:14646616 | pubmed:author | pubmed-author:HahmEunsilE | lld:pubmed |
| pubmed-article:14646616 | pubmed:author | pubmed-author:YangYoolheeY | lld:pubmed |
| pubmed-article:14646616 | pubmed:author | pubmed-author:ParkJong... | lld:pubmed |
| pubmed-article:14646616 | pubmed:author | pubmed-author:ParkHyunjeong... | lld:pubmed |
| pubmed-article:14646616 | pubmed:author | pubmed-author:PangSaicS | lld:pubmed |
| pubmed-article:14646616 | pubmed:author | pubmed-author:HurDaeyoungD | lld:pubmed |
| pubmed-article:14646616 | pubmed:author | pubmed-author:HwangYoung... | lld:pubmed |
| pubmed-article:14646616 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:14646616 | pubmed:volume | 13 | lld:pubmed |
| pubmed-article:14646616 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:14646616 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:14646616 | pubmed:pagination | 549-54 | lld:pubmed |
| pubmed-article:14646616 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
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| pubmed-article:14646616 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:14646616 | pubmed:articleTitle | Vitamin C downregulates interleukin-18 production by increasing reactive oxygen intermediate and mitogen-activated protein kinase signalling in B16F10 murine melanoma cells. | lld:pubmed |
| pubmed-article:14646616 | pubmed:affiliation | Department of Life Science, Sookmyung Women's University, Seoul, Korea. | lld:pubmed |
| pubmed-article:14646616 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:14646616 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:16173 | entrezgene:pubmed | pubmed-article:14646616 | lld:entrezgene |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:14646616 | lld:entrezgene |
