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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2003-12-3
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pubmed:abstractText |
We recently reported that interleukin-18 (IL-18) is highly expressed in malignant skin tumours such as melanomas, and may play a key role in the malignancy of such tumours. This study was designed to investigate the mechanisms of IL-18 regulation by vitamin C in B16F10 murine melanoma cells. Cells were treated with vitamin C, and the expression of IL-18 was measured by reverse transcription-polymerase chain reaction and intracellular flow cytometry analysis. Decreased IL-18 production and a significant reduction in IL-18 mRNA transcript were detected in cells treated with vitamin C. The effect of vitamin C treatment was blocked by the antioxidant N-acetyl-L-cysteine, suggesting that vitamin C affects IL-18 expression by up-regulating intracellular reactive oxygen intermediate (ROI) levels. To investigate whether the mitogen-activated protein kinase (MAPK) signalling pathway is involved in the downregulation of IL-18 production, cells were pretreated with SB203580, an inhibitor of p38 MAPK, prior to the addition of vitamin C. This pretreatment blocked the decrease in IL-18 production. However, vitamin C treatment enhanced the expression of phosphorylated p38 MAPK. Taken together, we conclude that vitamin C increases intracellular ROI levels, and regulates IL-18 production through the MAPK signalling pathway.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acetylcysteine,
http://linkedlifedata.com/resource/pubmed/chemical/Antioxidants,
http://linkedlifedata.com/resource/pubmed/chemical/Ascorbic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Imidazoles,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-18,
http://linkedlifedata.com/resource/pubmed/chemical/Pyridines,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Reactive Oxygen Species,
http://linkedlifedata.com/resource/pubmed/chemical/SB 203580,
http://linkedlifedata.com/resource/pubmed/chemical/p38 Mitogen-Activated Protein...
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0960-8931
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pubmed:author |
pubmed-author:ChoDaehoD,
pubmed-author:HahmEunsilE,
pubmed-author:HurDaeyoungD,
pubmed-author:HwangYoung IlYI,
pubmed-author:KangJae SeungJS,
pubmed-author:KimDaejinD,
pubmed-author:KimSeonghanS,
pubmed-author:KimYeong SeokYS,
pubmed-author:KimYoung-InYI,
pubmed-author:LeeWang JaeWJ,
pubmed-author:PangSaicS,
pubmed-author:ParkHyunjeongH,
pubmed-author:ParkJong HoonJH,
pubmed-author:YangYoolheeY
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pubmed:issnType |
Print
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pubmed:volume |
13
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
549-54
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:14646616-Acetylcysteine,
pubmed-meshheading:14646616-Animals,
pubmed-meshheading:14646616-Antioxidants,
pubmed-meshheading:14646616-Ascorbic Acid,
pubmed-meshheading:14646616-Blotting, Western,
pubmed-meshheading:14646616-Cell Line, Tumor,
pubmed-meshheading:14646616-Densitometry,
pubmed-meshheading:14646616-Down-Regulation,
pubmed-meshheading:14646616-Enzyme Inhibitors,
pubmed-meshheading:14646616-Flow Cytometry,
pubmed-meshheading:14646616-Imidazoles,
pubmed-meshheading:14646616-Immunoprecipitation,
pubmed-meshheading:14646616-Interleukin-18,
pubmed-meshheading:14646616-MAP Kinase Signaling System,
pubmed-meshheading:14646616-Melanoma,
pubmed-meshheading:14646616-Mice,
pubmed-meshheading:14646616-Pyridines,
pubmed-meshheading:14646616-RNA, Messenger,
pubmed-meshheading:14646616-Reactive Oxygen Species,
pubmed-meshheading:14646616-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:14646616-Signal Transduction,
pubmed-meshheading:14646616-Skin Neoplasms,
pubmed-meshheading:14646616-Time Factors,
pubmed-meshheading:14646616-p38 Mitogen-Activated Protein Kinases
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pubmed:year |
2003
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pubmed:articleTitle |
Vitamin C downregulates interleukin-18 production by increasing reactive oxygen intermediate and mitogen-activated protein kinase signalling in B16F10 murine melanoma cells.
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pubmed:affiliation |
Department of Life Science, Sookmyung Women's University, Seoul, Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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