Linked Life Data

14617572

Source:http://linkedlifedata.com/resource/pubmed/id/14617572

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2004-2-20
pubmed:abstractText
The neuropeptide vasoactive intestinal peptide (VIP) exerts its actions through two structurally related G protein-coupled receptors (VPAC(1) and VPAC(2)). Pituitary adenylate cyclase-activating polypeptide (PACAP) is also a potent agonist of VPAC(1) and VPAC(2) receptors as well as of a third, PACAP-specific receptor (PAC(1)). We report here the distribution of the VPAC(2) receptor in peripheral tissues of the mouse, determined by receptor autoradiography using [(125)I]VIP and the selective VPAC(2) receptor agonist [(125)I]Ro25-1553 in wild-type and VPAC(2) receptor-null mice. In addition, displacement experiments with the VPAC(2)-selective agonist Ro25-1553 and the VPAC(1)-selective agonist [K(15),R(16),L(27)]VIP(1-7)/GRF(8-27) were performed using the universal radioligand [(125)I]VIP. The VPAC(2) receptor is found predominantly in smooth muscle (in blood vessels and in the smooth muscle layers of the gastrointestinal and reproductive systems), the basal part of the mucosal epithelium in the colon, lung, the vasculature of the kidney, adrenal medulla, and retina. Unexpectedly, the receptor was also present in thyroid follicular cells and acinar cells of the pancreas, tissues that have not been found to express the receptor in other species, and in very large amounts in the lung. Our data suggest novel functions of the VPAC(2) receptor and additional potential therapeutic uses of drugs acting at the receptor (including the treatment of erectile dysfunction), but our results also indicate that caution should be exercised in using the mouse as an animal model for the evaluation of VIP analogs intended for diagnostic or therapeutic use in man.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0013-7227
pubmed:author
pubmed:issnType
Print
pubmed:volume
145
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1203-10
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:14617572-Animals, pubmed-meshheading:14617572-Autoradiography, pubmed-meshheading:14617572-Binding, Competitive, pubmed-meshheading:14617572-Blood Vessels, pubmed-meshheading:14617572-Digestive System, pubmed-meshheading:14617572-Endocrine System, pubmed-meshheading:14617572-Epithelium, pubmed-meshheading:14617572-Lymphatic System, pubmed-meshheading:14617572-Mice, pubmed-meshheading:14617572-Mice, Inbred C57BL, pubmed-meshheading:14617572-Mice, Mutant Strains, pubmed-meshheading:14617572-Muscle, Smooth, pubmed-meshheading:14617572-Peptide Fragments, pubmed-meshheading:14617572-Peptides, Cyclic, pubmed-meshheading:14617572-Radioligand Assay, pubmed-meshheading:14617572-Receptors, Vasoactive Intestinal Peptide, pubmed-meshheading:14617572-Receptors, Vasoactive Intestinal Peptide, Type II, pubmed-meshheading:14617572-Urogenital System, pubmed-meshheading:14617572-Vasoactive Intestinal Peptide
pubmed:year
2004
pubmed:articleTitle
Distribution of the VPAC2 receptor in peripheral tissues of the mouse.
pubmed:affiliation
Division of Neuroscience, School of Biomedical and Clinical Laboratory Sciences, University of Edinburgh, United Kingdom EH8 9JZ. tony.harmar@ed.ac.uk
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't