Linked Life Data

14609553

Source:http://linkedlifedata.com/resource/pubmed/id/14609553

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2003-11-11
pubmed:abstractText
Our understanding of MMP expression during corneal repair has previously relied upon animal models, isolated human biopsy specimens and cell culture studies. The aim of this study was to determine the temporal and spatial expression of matrix metalloproteinases following wounding of cultured human corneal tissue. Human corneas were cultured and cut into six pieces. The epithelium was removed with a corneal brush. The tissue was then re-cultured and tissue pieces were fixed up to 7 days post-wounding. Matrix metalloproteinases were detected by in situ hybridisation and immunohistochemistry. Intracellular laminin-5, a marker of migratory epithelial cells, was located immunohistologically. In the time scale studied tissue series from nine corneas achieved coverage of the stroma with epithelial cells and partial repair of damaged basement membrane, demonstrated by the Periodic acid-Schiff reaction and haematoxylin and eosin counter-staining. By day 3, migrating epithelial cells and stromal cells beneath the wounded area expressed collagenase-1 (MMP-1). Stromelysin-1 (MMP-3) was expressed only by fibroblast-like stromal cells. Stromelysin-2 (MMP-10) was detected in migrating epithelial cells and remained when the stroma was surrounded by a monolayer of epithelial cells. By day 7, development of multi-layered epithelium around the tissue coincided with cessation of MMP expression in both epithelial and stromal cells, except for MMP-9, which remained in epithelial basal cells. Tissue inhibitor of matrix metalloproteinase-1 was mainly associated with stromal cells and was reduced upon formation of a multi-layered epithelium. This study demonstrates matrix metalloproteinase expression in epithelial and fibroblast-like cells following wounding of human corneal tissue in culture where the cells remain in contact with their natural matrices.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0014-4835
pubmed:author
pubmed:issnType
Print
pubmed:volume
77
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
653-64
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:14609553-Basement Membrane, pubmed-meshheading:14609553-Cell Adhesion Molecules, pubmed-meshheading:14609553-Cell Movement, pubmed-meshheading:14609553-Collagenases, pubmed-meshheading:14609553-Cornea, pubmed-meshheading:14609553-Culture Techniques, pubmed-meshheading:14609553-Epithelial Cells, pubmed-meshheading:14609553-Humans, pubmed-meshheading:14609553-Immunohistochemistry, pubmed-meshheading:14609553-In Situ Hybridization, pubmed-meshheading:14609553-Matrix Metalloproteinase 10, pubmed-meshheading:14609553-Matrix Metalloproteinase 3, pubmed-meshheading:14609553-Matrix Metalloproteinase 9, pubmed-meshheading:14609553-Matrix Metalloproteinases, pubmed-meshheading:14609553-Metalloendopeptidases, pubmed-meshheading:14609553-Stromal Cells, pubmed-meshheading:14609553-Tissue Inhibitor of Metalloproteinase-1, pubmed-meshheading:14609553-Wound Healing
pubmed:year
2003
pubmed:articleTitle
Temporal and spatial expression of matrix metalloproteinases during wound healing of human corneal tissue.
pubmed:affiliation
Epithelial Repair and Regeneration Group, Wound Healing Research Unit, Division of Pathology, Institute of Ophthalmology, Bath Street, London EC1V 9EL, UK. j.daniels@ucl.ac.uk
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't