Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
12A
|
| pubmed:dateCreated |
1993-1-13
|
| pubmed:abstractText |
Recently, we reported the cloning of the Drosophila melanogaster homolog of the vertebrate CCAAT/enhancer-binding protein (C/EBP). Here, we describe studies of the DNA-binding and dimerization properties of Drosophila C/EBP (DmC/EBP), as well as its tissue distribution, developmental regulation, and essential role in embryonic development and conclude that it bears functional as well as structural similarity to mammalian C/EBP. DmC/EBP contains a basic region/leucine zipper (bZIP) DNA-binding domain very similar to that of mammalian C/EBP and the purified C/EBPs bound to DNA with the same sequence specificity. Among the DNA sequences that DmC/EBP bound with high affinity was a conserved site within the promoter of the DmC/EBP gene itself. In vitro, DmC/EBP and mammalian C/EBP specifically formed functional heterodimers; however, as we found no evidence for a family of DmC/EBPs, DmC/EBP may function as a homodimer in vivo. The DmC/EBP protein was expressed predominantly during late embryogenesis in the nuclei of a restricted set of differentiating cell types, such as the lining of the gut and epidermis, similar to the mammalian tissues that express C/EBP. We have characterized mutations in the DmC/EBP gene and found that deleting the gene caused late embryonic lethality. Embryos that lack C/EBP die just before or just upon hatching. The lethal phenotype of C/EBP mutants can be rescued with the cloned C/EBP gene introduced by P-element-mediated germ-line transformation. The strict requirement for C/EBP during Drosophila embryogenesis, coupled with its structural and functional similarities to mammalian C/EBP, provides a useful genetic system in which to study the role of C/EBP in development.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CCAAT-Enhancer-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0890-9369
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
6
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
2299-311
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:1459454-Amino Acid Sequence,
pubmed-meshheading:1459454-Animals,
pubmed-meshheading:1459454-Base Sequence,
pubmed-meshheading:1459454-Blotting, Northern,
pubmed-meshheading:1459454-CCAAT-Enhancer-Binding Proteins,
pubmed-meshheading:1459454-Cloning, Molecular,
pubmed-meshheading:1459454-DNA,
pubmed-meshheading:1459454-DNA-Binding Proteins,
pubmed-meshheading:1459454-Drosophila melanogaster,
pubmed-meshheading:1459454-Escherichia coli,
pubmed-meshheading:1459454-Germ Cells,
pubmed-meshheading:1459454-Molecular Sequence Data,
pubmed-meshheading:1459454-Nuclear Proteins,
pubmed-meshheading:1459454-Organ Specificity,
pubmed-meshheading:1459454-Promoter Regions, Genetic,
pubmed-meshheading:1459454-Protein Binding,
pubmed-meshheading:1459454-Restriction Mapping,
pubmed-meshheading:1459454-Sequence Homology, Amino Acid,
pubmed-meshheading:1459454-Transcription Factors,
pubmed-meshheading:1459454-Transformation, Genetic
|
| pubmed:year |
1992
|
| pubmed:articleTitle |
Drosophila C/EBP: a tissue-specific DNA-binding protein required for embryonic development.
|
| pubmed:affiliation |
Howard Hughes Research Laboratory, Department of Embryology, Carnegie Institution of Washington, Baltimore, Maryland 21210.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|