| pubmed-article:14588041 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:14588041 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:14588041 | lifeskim:mentions | umls-concept:C2347026 | lld:lifeskim |
| pubmed-article:14588041 | lifeskim:mentions | umls-concept:C0370003 | lld:lifeskim |
| pubmed-article:14588041 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:14588041 | lifeskim:mentions | umls-concept:C0030956 | lld:lifeskim |
| pubmed-article:14588041 | lifeskim:mentions | umls-concept:C0220825 | lld:lifeskim |
| pubmed-article:14588041 | lifeskim:mentions | umls-concept:C0201699 | lld:lifeskim |
| pubmed-article:14588041 | lifeskim:mentions | umls-concept:C0596495 | lld:lifeskim |
| pubmed-article:14588041 | lifeskim:mentions | umls-concept:C0936012 | lld:lifeskim |
| pubmed-article:14588041 | pubmed:issue | 21 | lld:pubmed |
| pubmed-article:14588041 | pubmed:dateCreated | 2003-10-31 | lld:pubmed |
| pubmed-article:14588041 | pubmed:abstractText | Although several designs have been advanced for coupling sample enrichment devices to a sheathless electrospray ionization-mass spectrometry (MS) interface on a capillary electrophoresis (CE) column, most of these approaches suffer from difficulties in fabrication, and the CE separation efficiency is degraded as a result of the presence of coupling sleeves. We have developed a design that offers significant improvements in terms of ease of fabrication, durability, and maintenance of the integrity of the CE-separated analyte zones. Capillaries with different inside and outside diameters were evaluated to optimize the performance of the CE-MS system, resulting in a mass limit of detection of 500 amol for tandem MS analysis of a standard peptide using a 20-microm-i.d. capillary. The improved design incorporates an efficient method to preconcentrate a sample directly within the CE capillary followed by its electrophoretic separation and detection using a true zero dead-volume sheathless CE-MS interface. Testing of this novel CE-MS system showed its ability to characterize proteomic samples such as protein digests, in-gel-digested proteins, and hydrophobic peptides as well as to quantitate ICAT-labeled peptides. | lld:pubmed |
| pubmed-article:14588041 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14588041 | pubmed:language | eng | lld:pubmed |
| pubmed-article:14588041 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14588041 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:14588041 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14588041 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14588041 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14588041 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14588041 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14588041 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14588041 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14588041 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14588041 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14588041 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14588041 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14588041 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14588041 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14588041 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:14588041 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:14588041 | pubmed:issn | 0003-2700 | lld:pubmed |
| pubmed-article:14588041 | pubmed:author | pubmed-author:ZhouMingM | lld:pubmed |
| pubmed-article:14588041 | pubmed:author | pubmed-author:ConradsThomas... | lld:pubmed |
| pubmed-article:14588041 | pubmed:author | pubmed-author:IssaqHaleem... | lld:pubmed |
| pubmed-article:14588041 | pubmed:author | pubmed-author:VeenstraTimot... | lld:pubmed |
| pubmed-article:14588041 | pubmed:author | pubmed-author:YuLi-RongLR | lld:pubmed |
| pubmed-article:14588041 | pubmed:author | pubmed-author:JaniniGeorge... | lld:pubmed |
| pubmed-article:14588041 | pubmed:author | pubmed-author:BlonderJosipJ | lld:pubmed |
| pubmed-article:14588041 | pubmed:author | pubmed-author:GignacMichell... | lld:pubmed |
| pubmed-article:14588041 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:14588041 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:14588041 | pubmed:volume | 75 | lld:pubmed |
| pubmed-article:14588041 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:14588041 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:14588041 | pubmed:pagination | 5984-93 | lld:pubmed |
| pubmed-article:14588041 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
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| pubmed-article:14588041 | pubmed:meshHeading | pubmed-meshheading:14588041... | lld:pubmed |
| pubmed-article:14588041 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:14588041 | pubmed:articleTitle | On-column sample enrichment for capillary electrophoresis sheathless electrospray ionization mass spectrometry: evaluation for peptide analysis and protein identification. | lld:pubmed |
| pubmed-article:14588041 | pubmed:affiliation | Laboratory of Proteomics and Analytical Technologies and Image Analysis Laboratory, SAIC-Frederick, Inc., National Cancer Institute at Frederick, P.O. Box B, Frederick, Maryland 21702, USA. Janini@mail.ncifcrf.gov | lld:pubmed |
| pubmed-article:14588041 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:14588041 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
