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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
21
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pubmed:dateCreated |
2003-10-31
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pubmed:abstractText |
Although several designs have been advanced for coupling sample enrichment devices to a sheathless electrospray ionization-mass spectrometry (MS) interface on a capillary electrophoresis (CE) column, most of these approaches suffer from difficulties in fabrication, and the CE separation efficiency is degraded as a result of the presence of coupling sleeves. We have developed a design that offers significant improvements in terms of ease of fabrication, durability, and maintenance of the integrity of the CE-separated analyte zones. Capillaries with different inside and outside diameters were evaluated to optimize the performance of the CE-MS system, resulting in a mass limit of detection of 500 amol for tandem MS analysis of a standard peptide using a 20-microm-i.d. capillary. The improved design incorporates an efficient method to preconcentrate a sample directly within the CE capillary followed by its electrophoretic separation and detection using a true zero dead-volume sheathless CE-MS interface. Testing of this novel CE-MS system showed its ability to characterize proteomic samples such as protein digests, in-gel-digested proteins, and hydrophobic peptides as well as to quantitate ICAT-labeled peptides.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Apoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Bacteriorhodopsins,
http://linkedlifedata.com/resource/pubmed/chemical/Cytochromes c,
http://linkedlifedata.com/resource/pubmed/chemical/Fibrinopeptide B,
http://linkedlifedata.com/resource/pubmed/chemical/Hydrofluoric Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Myoglobin,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Serum Albumin,
http://linkedlifedata.com/resource/pubmed/chemical/Silicon Dioxide,
http://linkedlifedata.com/resource/pubmed/chemical/Trypsin,
http://linkedlifedata.com/resource/pubmed/chemical/apomyoglobin
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0003-2700
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
75
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5984-93
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:14588041-Amino Acid Sequence,
pubmed-meshheading:14588041-Apoproteins,
pubmed-meshheading:14588041-Bacteriorhodopsins,
pubmed-meshheading:14588041-Cytochromes c,
pubmed-meshheading:14588041-Electrophoresis, Capillary,
pubmed-meshheading:14588041-Fibrinopeptide B,
pubmed-meshheading:14588041-Hydrofluoric Acid,
pubmed-meshheading:14588041-Hydrophobic and Hydrophilic Interactions,
pubmed-meshheading:14588041-Isotope Labeling,
pubmed-meshheading:14588041-Microscopy, Electron, Scanning,
pubmed-meshheading:14588041-Molecular Sequence Data,
pubmed-meshheading:14588041-Molecular Weight,
pubmed-meshheading:14588041-Myoglobin,
pubmed-meshheading:14588041-Peptide Fragments,
pubmed-meshheading:14588041-Peptides,
pubmed-meshheading:14588041-Proteins,
pubmed-meshheading:14588041-Serum Albumin,
pubmed-meshheading:14588041-Silicon Dioxide,
pubmed-meshheading:14588041-Spectrometry, Mass, Electrospray Ionization,
pubmed-meshheading:14588041-Trypsin
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pubmed:year |
2003
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pubmed:articleTitle |
On-column sample enrichment for capillary electrophoresis sheathless electrospray ionization mass spectrometry: evaluation for peptide analysis and protein identification.
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pubmed:affiliation |
Laboratory of Proteomics and Analytical Technologies and Image Analysis Laboratory, SAIC-Frederick, Inc., National Cancer Institute at Frederick, P.O. Box B, Frederick, Maryland 21702, USA. Janini@mail.ncifcrf.gov
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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