|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
Pt 22
|
|
pubmed:dateCreated |
2003-10-24
|
|
pubmed:abstractText |
The cellular antioxidant defense systems neutralize the cytotoxic by-products referred to as reactive oxygen species (ROS). Among them, selenoproteins have important antioxidant and detoxification functions. The interference in selenoprotein biosynthesis results in accumulation of ROS and consequently in a toxic intracellular environment. The resulting ROS imbalance can trigger apoptosis to eliminate the deleterious cells. In Drosophila, a null mutation in the selD gene (homologous to the human selenophosphate synthetase type 1) causes an impairment of selenoprotein biosynthesis, a ROS burst and lethality. We propose this mutation (known as selDptuf) as a tool to understand the link between ROS accumulation and cell death. To this aim we have analyzed the mechanism by which selDptuf mutant cells become apoptotic in Drosophila imaginal discs. The apoptotic effect of selDptuf does not require the activity of the Ras/MAPK-dependent proapoptotic gene hid, but results in stabilization of the tumor suppressor protein Dmp53 and transcription of the Drosophila pro-apoptotic gene reaper (rpr). We also provide genetic evidence that the initiator caspase DRONC is activated and that the effector caspase DRICE is processed to commit selDptuf mutant cells to death. Moreover, the ectopic expression of the inhibitor of apoptosis DIAP1 rescues the cellular viability of selDptuf mutant cells. These observations indicate that selDptuf ROS-induced apoptosis in Drosophila is mainly driven by the caspase-dependent Dmp53/Rpr pathway.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Caspases,
http://linkedlifedata.com/resource/pubmed/chemical/Drosophila Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Fungal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Ice protein, Drosophila,
http://linkedlifedata.com/resource/pubmed/chemical/Inhibitor of Apoptosis Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase...,
http://linkedlifedata.com/resource/pubmed/chemical/Nc protein, Drosophila,
http://linkedlifedata.com/resource/pubmed/chemical/Neuropeptides,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphotransferases,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Reactive Oxygen Species,
http://linkedlifedata.com/resource/pubmed/chemical/SelD protein, Drosophila,
http://linkedlifedata.com/resource/pubmed/chemical/Selenoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Suppressor Protein p53,
http://linkedlifedata.com/resource/pubmed/chemical/p53 protein, Drosophila,
http://linkedlifedata.com/resource/pubmed/chemical/ras Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/reaper protein, Drosophila,
http://linkedlifedata.com/resource/pubmed/chemical/selenophosphate synthetase,
http://linkedlifedata.com/resource/pubmed/chemical/thread protein, Drosophila,
http://linkedlifedata.com/resource/pubmed/chemical/wrinkled protein, Drosophila
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Nov
|
|
pubmed:issn |
0021-9533
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
15
|
|
pubmed:volume |
116
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
4597-604
|
|
pubmed:dateRevised |
2009-11-19
|
|
pubmed:meshHeading |
pubmed-meshheading:14576353-Animals,
pubmed-meshheading:14576353-Apoptosis,
pubmed-meshheading:14576353-Caspases,
pubmed-meshheading:14576353-Drosophila Proteins,
pubmed-meshheading:14576353-Drosophila melanogaster,
pubmed-meshheading:14576353-Eye,
pubmed-meshheading:14576353-Fungal Proteins,
pubmed-meshheading:14576353-Immunohistochemistry,
pubmed-meshheading:14576353-Inhibitor of Apoptosis Proteins,
pubmed-meshheading:14576353-Microscopy, Electron, Scanning,
pubmed-meshheading:14576353-Mitogen-Activated Protein Kinase Kinases,
pubmed-meshheading:14576353-Mutation,
pubmed-meshheading:14576353-Neuropeptides,
pubmed-meshheading:14576353-Phosphotransferases,
pubmed-meshheading:14576353-Protein Binding,
pubmed-meshheading:14576353-Proteins,
pubmed-meshheading:14576353-Reactive Oxygen Species,
pubmed-meshheading:14576353-Selenoproteins,
pubmed-meshheading:14576353-Trans-Activators,
pubmed-meshheading:14576353-Tumor Suppressor Protein p53,
pubmed-meshheading:14576353-ras Proteins
|
|
pubmed:year |
2003
|
|
pubmed:articleTitle |
DIAP1 suppresses ROS-induced apoptosis caused by impairment of the selD/sps1 homolog in Drosophila.
|
|
pubmed:affiliation |
Departament de Genètica, Facultat de Biologia, Universitat de Barcelona, Diagonal 645, 08028 Barcelona, Spain.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
