| pubmed-article:14560570 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:14560570 | lifeskim:mentions | umls-concept:C0034861 | lld:lifeskim |
| pubmed-article:14560570 | lifeskim:mentions | umls-concept:C0067033 | lld:lifeskim |
| pubmed-article:14560570 | lifeskim:mentions | umls-concept:C0004358 | lld:lifeskim |
| pubmed-article:14560570 | lifeskim:mentions | umls-concept:C0264939 | lld:lifeskim |
| pubmed-article:14560570 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:14560570 | pubmed:dateCreated | 2003-10-16 | lld:pubmed |
| pubmed-article:14560570 | pubmed:abstractText | The presence of antineutrophil cytoplasmic autoantibodies with specificity for proteinase 3 (PR3-ANCA) usually is detected by enzyme-linked immunosorbent assay (ELISA) with purified PR3 as a substrate. We studied the technical performance of direct and capture ELISA using a recombinant proteolytically inactive form of PR3 produced in the baculovirus expression system for the detection of PR3-ANCA in 114 patients with systemic vasculitis at diagnosis. We found that ELISA using recombinant PR3 produced in insect cells is a promising alternative for ELISA with native PR3. We found a correlation between tests using recombinant or native PR3, as well as correlation of the ELISA results with ANCA titers measured by the indirect immunofluorescence technique. However, the specificity for ANCA-associated vasculitis of ELISA with recombinant PR3 was lower than ELISA using native PR3. Compared with the direct assay, capture ELISA is a more sensitive method for PR3-ANCA detection, with both native and recombinant PR3, and its results depend on the monoclonal antibody used to capture the antigen. | lld:pubmed |
| pubmed-article:14560570 | pubmed:language | eng | lld:pubmed |
| pubmed-article:14560570 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14560570 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:14560570 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14560570 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14560570 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14560570 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14560570 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14560570 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14560570 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:14560570 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:14560570 | pubmed:issn | 0002-9173 | lld:pubmed |
| pubmed-article:14560570 | pubmed:author | pubmed-author:StegemanCoen... | lld:pubmed |
| pubmed-article:14560570 | pubmed:author | pubmed-author:LimburgPieter... | lld:pubmed |
| pubmed-article:14560570 | pubmed:author | pubmed-author:RarokAgnieszk... | lld:pubmed |
| pubmed-article:14560570 | pubmed:author | pubmed-author:SchmittJackyJ | lld:pubmed |
| pubmed-article:14560570 | pubmed:author | pubmed-author:HuitemaMinke... | lld:pubmed |
| pubmed-article:14560570 | pubmed:author | pubmed-author:van der... | lld:pubmed |
| pubmed-article:14560570 | pubmed:author | pubmed-author:van der... | lld:pubmed |
| pubmed-article:14560570 | pubmed:author | pubmed-author:BertholdHeike... | lld:pubmed |
| pubmed-article:14560570 | pubmed:author | pubmed-author:KallenbergCee... | lld:pubmed |
| pubmed-article:14560570 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:14560570 | pubmed:volume | 120 | lld:pubmed |
| pubmed-article:14560570 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:14560570 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:14560570 | pubmed:pagination | 586-95 | lld:pubmed |
| pubmed-article:14560570 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:14560570 | pubmed:meshHeading | pubmed-meshheading:14560570... | lld:pubmed |
| pubmed-article:14560570 | pubmed:meshHeading | pubmed-meshheading:14560570... | lld:pubmed |
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| pubmed-article:14560570 | pubmed:meshHeading | pubmed-meshheading:14560570... | lld:pubmed |
| pubmed-article:14560570 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:14560570 | pubmed:articleTitle | Recombinant protein to analyze autoantibodies to proteinase 3 in systemic vasculitis. | lld:pubmed |
| pubmed-article:14560570 | pubmed:affiliation | Department of Internal Medicine, University Hospital Groningen, Hanzeplein 1, 9713 GZ Groningen, The Netherlands. | lld:pubmed |
| pubmed-article:14560570 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:14560570 | pubmed:publicationType | Comparative Study | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:14560570 | lld:pubmed |
