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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2003-10-16
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pubmed:abstractText |
The presence of antineutrophil cytoplasmic autoantibodies with specificity for proteinase 3 (PR3-ANCA) usually is detected by enzyme-linked immunosorbent assay (ELISA) with purified PR3 as a substrate. We studied the technical performance of direct and capture ELISA using a recombinant proteolytically inactive form of PR3 produced in the baculovirus expression system for the detection of PR3-ANCA in 114 patients with systemic vasculitis at diagnosis. We found that ELISA using recombinant PR3 produced in insect cells is a promising alternative for ELISA with native PR3. We found a correlation between tests using recombinant or native PR3, as well as correlation of the ELISA results with ANCA titers measured by the indirect immunofluorescence technique. However, the specificity for ANCA-associated vasculitis of ELISA with recombinant PR3 was lower than ELISA using native PR3. Compared with the direct assay, capture ELISA is a more sensitive method for PR3-ANCA detection, with both native and recombinant PR3, and its results depend on the monoclonal antibody used to capture the antigen.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0002-9173
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
120
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
586-95
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:14560570-Animals,
pubmed-meshheading:14560570-Antibodies, Antineutrophil Cytoplasmic,
pubmed-meshheading:14560570-Antibodies, Monoclonal,
pubmed-meshheading:14560570-Cells, Cultured,
pubmed-meshheading:14560570-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:14560570-Female,
pubmed-meshheading:14560570-Fluorescent Antibody Technique, Indirect,
pubmed-meshheading:14560570-Genetic Vectors,
pubmed-meshheading:14560570-Humans,
pubmed-meshheading:14560570-Male,
pubmed-meshheading:14560570-Myeloblastin,
pubmed-meshheading:14560570-Recombinant Proteins,
pubmed-meshheading:14560570-Sensitivity and Specificity,
pubmed-meshheading:14560570-Serine Endopeptidases,
pubmed-meshheading:14560570-Spodoptera,
pubmed-meshheading:14560570-Vasculitis,
pubmed-meshheading:14560570-Viral Proteins
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pubmed:year |
2003
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pubmed:articleTitle |
Recombinant protein to analyze autoantibodies to proteinase 3 in systemic vasculitis.
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pubmed:affiliation |
Department of Internal Medicine, University Hospital Groningen, Hanzeplein 1, 9713 GZ Groningen, The Netherlands.
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pubmed:publicationType |
Journal Article,
Comparative Study
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