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rdf:type |
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lifeskim:mentions |
umls-concept:C0002345,
umls-concept:C0017337,
umls-concept:C0019704,
umls-concept:C0023675,
umls-concept:C0026336,
umls-concept:C0086418,
umls-concept:C0218349,
umls-concept:C0292369,
umls-concept:C0376249,
umls-concept:C0679823,
umls-concept:C0812273,
umls-concept:C1336678,
umls-concept:C1366903,
umls-concept:C1418793,
umls-concept:C1424624
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pubmed:issue |
2
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pubmed:dateCreated |
2003-10-3
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pubmed:databankReference |
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pubmed:abstractText |
Alternative splicing may generate splice forms with different biologic roles or missing protein domains implicated in the interaction with HIV-1. To address this issue, 6 human genes were investigated-tumor suppressor gene 101 (TSG101), beta-transducin repeats containing protein (betaTrCP), peptidyl-proly cis-trans isomerase, cyclophilin A (PPIA), integrase interactor 1 protein (INI1), Nef-associated factor 1 (NAF1), and promyelacytic leukemia (PML)-involved in the viral life cycle and HIV-1 pathogenesis. All 6 genes presented alternative splicing, and a combined bioinformatic and reverse transcription polymerase chain reaction (RT-PCR) analysis identified 27 new variants for a total of 53 splice forms (an average of 9 variants per gene). The predicted frequency of the various splice forms based on expressed sequence tags (EST) analysis corresponded to the semiquantitative findings on RT-PCR analysis for the cell culture systems and for native CD4 cells investigated. Interindividual variation in the frequencies of various splice forms in CD4 T cells from blood donors was observed for INI1. Cell type-specific variation of splice pattern was observed for NAF1. Eight splice forms lacked or modified motifs implicated in the interaction with HIV-1, underscoring the potential interest of assessing alternative splicing when investigating viral cell biology and pathogenesis.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/BTRC protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Chromosomal Proteins, Non-Histone,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclophilin A,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Endosomal Sorting Complexes...,
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/PML protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/SMARCB1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/TNIP1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Tsg101 protein,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Suppressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/beta-Transducin Repeat-Containing...
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1525-4135
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
34
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
127-33
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pubmed:dateRevised |
2010-9-10
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pubmed:meshHeading |
pubmed-meshheading:14526201-Alternative Splicing,
pubmed-meshheading:14526201-Base Sequence,
pubmed-meshheading:14526201-Chromosomal Proteins, Non-Histone,
pubmed-meshheading:14526201-Computational Biology,
pubmed-meshheading:14526201-Cyclophilin A,
pubmed-meshheading:14526201-DNA-Binding Proteins,
pubmed-meshheading:14526201-Endosomal Sorting Complexes Required for Transport,
pubmed-meshheading:14526201-GTP-Binding Proteins,
pubmed-meshheading:14526201-Gene Frequency,
pubmed-meshheading:14526201-HIV-1,
pubmed-meshheading:14526201-Humans,
pubmed-meshheading:14526201-Molecular Sequence Data,
pubmed-meshheading:14526201-Neoplasm Proteins,
pubmed-meshheading:14526201-Nuclear Proteins,
pubmed-meshheading:14526201-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:14526201-Transcription Factors,
pubmed-meshheading:14526201-Tumor Suppressor Proteins,
pubmed-meshheading:14526201-beta-Transducin Repeat-Containing Proteins
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pubmed:year |
2003
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pubmed:articleTitle |
High frequency of alternative splicing of human genes participating in the HIV-1 life cycle: a model using TSG101, betaTrCP, PPIA, INI1, NAF1, and PML.
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pubmed:affiliation |
Division of Infectious Diseases and Institute of Microbiology, University Hospital of Lausanne, Switzerland.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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