Linked Life Data

14516168

Source:http://linkedlifedata.com/resource/pubmed/id/14516168

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2003-9-30
pubmed:abstractText
In this study, bovine viral diarrhoea virus (BVDV) was detected and bio-typically characterised in clinical samples using reverse transcriptase-polymerase chain reaction (RT-PCR). The RT-PCR technique produced two different amplicons (402 and approx. 680 bp in size) in case of the presence of both biotypes (cp and ncp) in the sample. The mixture of the biotypes as detected by RT-PCR was verified by the immunoplaque assay (IPA). Purification of biotypes was carried out by native plaque isolation and subsequent RT-PCR revealed single products (402 or approx. 680 bp in size) in each clone. The results showed that RT-PCR can be used for accurate molecular differentiation between the BVDV biotypes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0236-6290
pubmed:author
pubmed:issnType
Print
pubmed:volume
51
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
425-31
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Biotypic characterisation of bovine viral diarrhoea virus (BVDV) using reverse transcription-polymerase chain reaction (RT-PCR) in clinical samples.
pubmed:affiliation
Department of Virology, Faculty of Veterinary Medicine, Ankara University, 06110 Ankara, Turkey. karaoglu@veterinary.ankara.edu.tr
pubmed:publicationType
Journal Article, Evaluation Studies