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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5641
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pubmed:dateCreated |
2003-9-26
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pubmed:abstractText |
The RNA interference (RNAi) pathway is initiated by processing long double-stranded RNA into small interfering RNA (siRNA). The siRNA-generating enzyme was purified from Drosophila S2cells and consists of two stoichiometric subunits: Dicer-2(DCR-2) and a previously unknown protein that we named R2D2. R2D2 is homologous to the Caenorhabditis elegans RNAi protein RDE-4. Association with R2D2 does not affect the enzymatic activity of DCR-2. Rather, the DCR-2/R2D2 complex, but not DCR-2 alone, binds to siRNA and enhances sequence-specific messenger RNA degradation mediated by the RNA-initiated silencing complex (RISC). These results indicate that R2D2 bridges the initiation and effector steps of the Drosophila RNAi pathway by facilitating siRNA passage from Dicer to RISC.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/AGO2 protein, Drosophila,
http://linkedlifedata.com/resource/pubmed/chemical/Argonaute Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Caenorhabditis elegans Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Drosophila Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Endoribonucleases,
http://linkedlifedata.com/resource/pubmed/chemical/RDE-4 protein, C elegans,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Double-Stranded,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Interfering,
http://linkedlifedata.com/resource/pubmed/chemical/RNA Helicases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA-Induced Silencing Complex,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Ribonuclease III
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1095-9203
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
26
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pubmed:volume |
301
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1921-5
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:14512631-Amino Acid Sequence,
pubmed-meshheading:14512631-Animals,
pubmed-meshheading:14512631-Argonaute Proteins,
pubmed-meshheading:14512631-Biotinylation,
pubmed-meshheading:14512631-Caenorhabditis elegans,
pubmed-meshheading:14512631-Caenorhabditis elegans Proteins,
pubmed-meshheading:14512631-Cell Line,
pubmed-meshheading:14512631-Chemical Precipitation,
pubmed-meshheading:14512631-Drosophila Proteins,
pubmed-meshheading:14512631-Drosophila melanogaster,
pubmed-meshheading:14512631-Electrophoretic Mobility Shift Assay,
pubmed-meshheading:14512631-Endoribonucleases,
pubmed-meshheading:14512631-Kinetics,
pubmed-meshheading:14512631-Molecular Sequence Data,
pubmed-meshheading:14512631-Mutation,
pubmed-meshheading:14512631-Protein Structure, Tertiary,
pubmed-meshheading:14512631-RNA, Double-Stranded,
pubmed-meshheading:14512631-RNA, Messenger,
pubmed-meshheading:14512631-RNA, Small Interfering,
pubmed-meshheading:14512631-RNA Helicases,
pubmed-meshheading:14512631-RNA Interference,
pubmed-meshheading:14512631-RNA-Binding Proteins,
pubmed-meshheading:14512631-RNA-Induced Silencing Complex,
pubmed-meshheading:14512631-Recombinant Proteins,
pubmed-meshheading:14512631-Ribonuclease III
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pubmed:year |
2003
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pubmed:articleTitle |
R2D2, a bridge between the initiation and effector steps of the Drosophila RNAi pathway.
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pubmed:affiliation |
Howard Hughes Medical Institute and Department of Biochemistry, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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