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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2003-9-26
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pubmed:abstractText |
It is in dispute whether caspase 3 contributes to status epilepticus (SE)-induced cell loss. We hypothesized that caspase 3-mediated cell death continues beyond the acute phase of SE. We induced SE with either kainic acid or electrical stimulation of the amygdala in Wistar and Sprague-Dawley rats. Caspase 3 immunohistochemistry, Western blot analysis and enzyme activity measurements were used to determine cellular localization and the time course of caspase 3 expression and activation. Immunohistochemistry indicated that caspase 3 protein expression increased following SE, peaking at 16-24 h. Cleavage of procaspase 3 to active fragments (p20-17) was detected 2-7 days after SE. Caspase 3 enzyme activity was elevated at 8 h and further increased up to 19.4-fold at 7 days following SE. Activation of caspase 3 after SE occurred in the hippocampus and the extrahippocampal temporal lobe but not in the thalamus. Caspase 3-immunoreactive cells represented only a minority of degenerating cells as assessed by Fluoro-Jade B and TUNEL staining. Analysis of double-labelled sections indicated that active caspase 3 was located in astrocytes rather than neurons or microglia. There was increased caspase 3 expression in both rat strains, and it was independent of the method used to induce SE. These data demonstrate that caspase 3 contributes to the cell death occurring within the first week after SE, but in only a small proportion of degenerating cells. These results suggest that, contrary to expectations, caspase 3 inhibitors would have only limited benefits in the treatment of SE.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Casp3 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Caspase 3,
http://linkedlifedata.com/resource/pubmed/chemical/Caspases,
http://linkedlifedata.com/resource/pubmed/chemical/DEVDase,
http://linkedlifedata.com/resource/pubmed/chemical/Excitatory Amino Acid Agonists,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/Glial Fibrillary Acidic Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Kainic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Organic Chemicals,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Hydrolases,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphopyruvate Hydratase,
http://linkedlifedata.com/resource/pubmed/chemical/fluoro jade
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0953-816X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
18
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1486-96
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:14511328-Animals,
pubmed-meshheading:14511328-Blotting, Western,
pubmed-meshheading:14511328-Brain,
pubmed-meshheading:14511328-Caspase 3,
pubmed-meshheading:14511328-Caspases,
pubmed-meshheading:14511328-Cell Count,
pubmed-meshheading:14511328-Cell Death,
pubmed-meshheading:14511328-Disease Models, Animal,
pubmed-meshheading:14511328-Electric Stimulation,
pubmed-meshheading:14511328-Enzyme Activation,
pubmed-meshheading:14511328-Excitatory Amino Acid Agonists,
pubmed-meshheading:14511328-Fluorescent Dyes,
pubmed-meshheading:14511328-Glial Fibrillary Acidic Protein,
pubmed-meshheading:14511328-Immunohistochemistry,
pubmed-meshheading:14511328-In Situ Nick-End Labeling,
pubmed-meshheading:14511328-Kainic Acid,
pubmed-meshheading:14511328-Male,
pubmed-meshheading:14511328-Neuroglia,
pubmed-meshheading:14511328-Organic Chemicals,
pubmed-meshheading:14511328-Peptide Fragments,
pubmed-meshheading:14511328-Peptide Hydrolases,
pubmed-meshheading:14511328-Phosphopyruvate Hydratase,
pubmed-meshheading:14511328-Rats,
pubmed-meshheading:14511328-Rats, Sprague-Dawley,
pubmed-meshheading:14511328-Status Epilepticus,
pubmed-meshheading:14511328-Time Factors
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pubmed:year |
2003
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pubmed:articleTitle |
Expression and activation of caspase 3 following status epilepticus in the rat.
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pubmed:affiliation |
A.I. Virtanen Institute for Molecular Sciences, University of Kuopio, PO Box 1627, FIN-70 211 Kuopio, Finland.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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