Linked Life Data

14499953

Source:http://linkedlifedata.com/resource/pubmed/id/14499953

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2003-9-22
pubmed:abstractText
N-Methyl-D-aspartate (NMDA) receptors are a subtype of glutamate receptor that serve important functions at glutamatergic synapses in the brain. NMDA receptors are inhibited by concentrations of ethanol that are associated with intoxication and chronic exposure of neurons to ethanol enhances NMDA receptor function. The factors that underlie the acute inhibition of NMDA receptors by ethanol are not completely known, but ethanol sensitivity is influenced by receptor subunit composition. In this study, the effect of the regulatory subunit, NR3, on ethanol inhibition of NMDA receptors was examined. Recombinant NMDA receptors comprised of NR1 and NR2 (A-D) subunits were transiently transfected into HEK293 cells in the absence or presence of the NR3 subunit. In the absence of NR3, all NMDA receptor subunit combinations were inhibited by 100 mM ethanol. Co-expression of NR3 or an NR3-GFP fusion protein with NR1/NR2 (A-D) subunits did not alter the inhibitory effects of ethanol. In addition, the inhibition of NR1/NR2B receptors by the NR2B subunit-selective antagonist, ifenprodil, was not altered by co-expression of the NR3 subunit. Overall, these results suggest that the NR3A subunit is not a determinant of ethanol sensitivity in recombinant NMDA receptors.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0006-8993
pubmed:author
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
987
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
117-21
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Effect of the NR3 subunit on ethanol inhibition of recombinant NMDA receptors.
pubmed:affiliation
Department of Physiology and Neuroscience, Center for Drug and Alcohol Programs, Medical University of South Carolina, Charleston, SC 29425, USA. smotherc@musc.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.