Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1965-5-1
pubmed:abstractText
Smull, Christine E. (The Geisinger Medical Center, Danville, Pa.), and E. H. Ludwig. Infectivity of poliovirus and its nucleic acid for dehydrated HeLa cell monolayers. J. Bacteriol. 89:52-57. 1965.-A study was made of the infectivity of poliovirus ribonucleic acid (RNA), applied in various diluents, and of poliovirus, on cell monolayers which were washed free from their nutrient medium and allowed to dehydrate for periods up to 3 days prior to inoculation. The plaque formation of poliovirus RNA, applied in isotonic diluent, was greatly increased when assayed on cell monolayers dehydrated for certain periods of time. Conditions are described for bringing about optimal plaque formation by this means, and evidence is presented which strongly indicates that dehydration of the cell is an important factor in the increase in plaque formation of the poliovirus RNA. The plaque formation of poliovirus RNA in certain hypertonic diluents and in a basic protein-containing diluent was also improved with the use of dehydrated cell monolayers, whereas the plaque formation of poliovirus varied very little when assayed on cell monolayers dehydrated for periods up to 2 days. Under certain conditions, the size of the plaques increased as the dehydration time of the cell monolayers was increased. This was true with plaques initiated either by poliovirus or the nucleic acid. Further investigation in these cases revealed that virus production at given times was considerably greater in the dehydrated than in the undried cell monolayers.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/14255681-13437604, http://linkedlifedata.com/resource/pubmed/commentcorrection/14255681-13575680, http://linkedlifedata.com/resource/pubmed/commentcorrection/14255681-13639534, http://linkedlifedata.com/resource/pubmed/commentcorrection/14255681-13715281, http://linkedlifedata.com/resource/pubmed/commentcorrection/14255681-13724573, http://linkedlifedata.com/resource/pubmed/commentcorrection/14255681-13729565, http://linkedlifedata.com/resource/pubmed/commentcorrection/14255681-13802073, http://linkedlifedata.com/resource/pubmed/commentcorrection/14255681-13819961, http://linkedlifedata.com/resource/pubmed/commentcorrection/14255681-13889896, http://linkedlifedata.com/resource/pubmed/commentcorrection/14255681-13989646, http://linkedlifedata.com/resource/pubmed/commentcorrection/14255681-14047226, http://linkedlifedata.com/resource/pubmed/commentcorrection/14255681-14137640, http://linkedlifedata.com/resource/pubmed/commentcorrection/14255681-14402608, http://linkedlifedata.com/resource/pubmed/commentcorrection/14255681-14410319
pubmed:keyword
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
OM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
89
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
52-7
pubmed:dateRevised
2011-9-28
pubmed:meshHeading
pubmed:year
1965
pubmed:articleTitle
INFECTIVITY OF POLIOVIRUS AND ITS NUCLEIC ACID FOR DEHYDRATED HELA CELL MONOLAYERS.
pubmed:publicationType
Journal Article