Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1992-12-22
pubmed:abstractText
There is a high level of conservation between human chromosomes and bovine syntenic groups. One such comparison is between human chromosome 12 and bovine chromosome 5, where at least 16 loci have been shown to be conserved in an homologous segment. However, the degree of conservation of order of the loci on bovine chromosome 5 is unknown, and in general the conservation of order in comparisons between humans and cattle can only be speculated. We have estimated the recombination fractions between five of the loci that were previously published as mapping to bovine chromosome 5 by a combination of in situ hybridization and analysis of bovine-rodent somatic cell hybrid lines to determine whether order has been conserved in the homologous segment of bovine chromosome 5 and human chromosome 12. Recombination fractions were estimated in reference pedigrees of cattle. The loci were A2M, GSNL, HOX3, INT1, KRAS2, and PAH. Restriction fragment length polymorphisms for all loci were defined by screening a panel of eight restriction endonucleases. The linkage between loci was estimated using the lod score method, and all possible pairwise comparisons were made. A preliminary map was created by joining together loci that showed the smallest recombination fractions and the largest lod scores. A multipoint analysis was performed to estimate support for the most likely order. This order shows the relative inversion of some of the loci. Moreover, the distance spanned in cattle is less than a quarter the distance spanned in humans. Together, these data indicate that several chromosomal evolutionary events have occurred in the homologous segment shared by humans and cattle.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0888-7543
pubmed:author
pubmed:issnType
Print
pubmed:volume
14
pubmed:geneSymbol
A2M, INT1, KRAS2, PAH
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
38-42
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:1385300-Animals, pubmed-meshheading:1385300-Blotting, Southern, pubmed-meshheading:1385300-Cattle, pubmed-meshheading:1385300-Chromosome Mapping, pubmed-meshheading:1385300-DNA Probes, pubmed-meshheading:1385300-Female, pubmed-meshheading:1385300-Genes, Homeobox, pubmed-meshheading:1385300-Genes, ras, pubmed-meshheading:1385300-Genetic Linkage, pubmed-meshheading:1385300-Lod Score, pubmed-meshheading:1385300-Male, pubmed-meshheading:1385300-Nucleic Acid Hybridization, pubmed-meshheading:1385300-Phenylalanine Hydroxylase, pubmed-meshheading:1385300-Polymorphism, Restriction Fragment Length, pubmed-meshheading:1385300-Proto-Oncogene Proteins, pubmed-meshheading:1385300-Recombination, Genetic, pubmed-meshheading:1385300-Wnt Proteins, pubmed-meshheading:1385300-Zebrafish Proteins, pubmed-meshheading:1385300-alpha-Macroglobulins
pubmed:year
1992
pubmed:articleTitle
Linkage relations between A2M, HOX3, INT1, KRAS2, and PAH on bovine chromosome 5.
pubmed:affiliation
CSIRO, Division of Tropical Animal Production, Gehrmann Laboratories, Ritchie Research Complex, University of Queensland, Australia.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't