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rdf:type |
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lifeskim:mentions |
umls-concept:C0007589,
umls-concept:C0017262,
umls-concept:C0017337,
umls-concept:C0018183,
umls-concept:C0079459,
umls-concept:C0080347,
umls-concept:C0185117,
umls-concept:C0871261,
umls-concept:C0887899,
umls-concept:C1511938,
umls-concept:C1533157,
umls-concept:C1704632,
umls-concept:C1706817,
umls-concept:C2911684,
umls-concept:C2911692
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pubmed:issue |
1
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pubmed:dateCreated |
1992-2-10
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pubmed:abstractText |
Expression of the Evi-1 gene is frequently activated in murine myeloid leukemias by retroviral insertions immediately 5' or 90 kb 5' of the gene. The Evi-1 gene product is a nuclear, DNA-binding zinc finger protein of 145 kDa. On the basis of the properties of the myeloid cell lines in which the Evi-1 gene is activated, it has been hypothesized that its expression blocks normal differentiation. To explore this proposed role, we have constructed a retrovirus vector containing the gene and examined its effects on an interleukin-3-dependent myeloid cell line that differentiates in response to granulocyte colony-stimulating factor (G-CSF). Expression of the Evi-1 gene in these cells did not alter the normal growth factor requirements of the cells. However, expression of the Evi-1 gene blocked the ability of the cells to express myeloperoxidase and to terminally differentiate to granulocytes in response to G-CSF. This effect was not due to altered expression of the G-CSF receptor or to changes in the initial responses of the cells to G-CSF. These results support the hypothesis that the inappropriate expression of the Evi-1 gene in myeloid cells interferes with the ability of the cells to terminally differentiate.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1370341-1699199,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1370341-1705687,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1370341-1705688,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1370341-1848663,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1370341-1893871,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1370341-2017172,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1370341-2106070,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1370341-2107491,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1370341-2158039,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1370341-2158861,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1370341-2463930,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1370341-2468124,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1370341-2480562,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1370341-2542863,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1370341-2582266,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1370341-2668305,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1370341-2827004,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1370341-2842066,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1370341-2897103,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1370341-3014527,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1370341-3029127,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1370341-3259302,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1370341-3317408,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1370341-3785217,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1370341-4705382,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1370341-6574462,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1370341-6678608,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1370341-6809821
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0270-7306
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
12
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pubmed:geneSymbol |
Evi-1
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
183-9
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pubmed:dateRevised |
2010-9-7
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pubmed:meshHeading |
pubmed-meshheading:1370341-Animals,
pubmed-meshheading:1370341-Cell Differentiation,
pubmed-meshheading:1370341-Cell Division,
pubmed-meshheading:1370341-Cell Transformation, Neoplastic,
pubmed-meshheading:1370341-DNA-Binding Proteins,
pubmed-meshheading:1370341-Gene Expression,
pubmed-meshheading:1370341-Granulocyte Colony-Stimulating Factor,
pubmed-meshheading:1370341-Granulocytes,
pubmed-meshheading:1370341-Leukemia, Myeloid,
pubmed-meshheading:1370341-Mice,
pubmed-meshheading:1370341-Proto-Oncogenes,
pubmed-meshheading:1370341-Transcription Factors,
pubmed-meshheading:1370341-Tumor Cells, Cultured,
pubmed-meshheading:1370341-Zinc Fingers
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pubmed:year |
1992
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pubmed:articleTitle |
Expression of the Evi-1 zinc finger gene in 32Dc13 myeloid cells blocks granulocytic differentiation in response to granulocyte colony-stimulating factor.
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pubmed:affiliation |
Department of Biochemistry, St. Jude Children's Research Hospital, Memphis, Tennessee 38105.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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