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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1993-1-6
|
| pubmed:abstractText |
To study the phenotypic specificity of S-100 beta and insulin-like growth factor II (IGF-II) for developing monoamine neurons, serotonin (5-HT) neurons from the embryonic day 14 (E14) rostral raphe or dopamine (TH) neurons from the substantia nigra/ventral tegmental area were cultured for 3 days in vitro (3 DIV) in the presence of these factors. Neuronotrophic effects were analyzed by computer-assisted morphometry of 5-HT and TH-immunoreactive neurons. S-100 beta and IGF-II differentially regulated the growth of 5-HT and TH neurons but did not affect their survival. S-100 beta significantly increased several parameters of neurite outgrowth by 5-HT neurons but inhibited the spatial extent (field area) of TH neurites. IGF-II promoted growth of cell bodies of both phenotype, but only stimulated neurite outgrowth by TH neurons. S-100 beta and IGF-II differentially affected the number of GFAP immunoreactive cells from raphe and substantia nigra, but these effects did not correlate with the specificity of neuronotrophic effects. S-100 beta and IGF-II immunoreactivities were expressed in glial cultures derived from the same brain regions, raising the possibility that these factors have autocrine effects on glia as well as paracrine actions on neurons. The results of this study suggest that specificity of neurotrophic factors for particular embryonic neurons may be correlated with their neurotransmitter phenotype.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Glial Fibrillary Acidic Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin-Like Growth Factor II,
http://linkedlifedata.com/resource/pubmed/chemical/S100 Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Serotonin,
http://linkedlifedata.com/resource/pubmed/chemical/Tyrosine 3-Monooxygenase
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0360-4012
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
33
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
248-56
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:1360544-Animals,
pubmed-meshheading:1360544-Cell Division,
pubmed-meshheading:1360544-Cells, Cultured,
pubmed-meshheading:1360544-Dopamine,
pubmed-meshheading:1360544-Embryo, Mammalian,
pubmed-meshheading:1360544-Glial Fibrillary Acidic Protein,
pubmed-meshheading:1360544-Immunohistochemistry,
pubmed-meshheading:1360544-Insulin-Like Growth Factor II,
pubmed-meshheading:1360544-Neurites,
pubmed-meshheading:1360544-Neuroglia,
pubmed-meshheading:1360544-Neurons,
pubmed-meshheading:1360544-Raphe Nuclei,
pubmed-meshheading:1360544-Rats,
pubmed-meshheading:1360544-S100 Proteins,
pubmed-meshheading:1360544-Serotonin,
pubmed-meshheading:1360544-Substantia Nigra,
pubmed-meshheading:1360544-Tegmentum Mesencephali,
pubmed-meshheading:1360544-Tyrosine 3-Monooxygenase
|
| pubmed:year |
1992
|
| pubmed:articleTitle |
S-100 beta and insulin-like growth factor-II differentially regulate growth of developing serotonin and dopamine neurons in vitro.
|
| pubmed:affiliation |
Department of Cell Biology and Anatomy, University of North Carolina School of Medicine, Chapel Hill 27599-7090.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|