Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
10
|
pubmed:dateCreated |
1992-8-31
|
pubmed:abstractText |
The effect of variations in Neisseria meningitidis pili on bacterial interactions with three epithelial cell lines as well as human umbilical vein endothelial cells was studied using a panel of seven strains expressing Class I or Class II pili. Comparison of adherence of piliated and pilus-deficient variants of each strain to epithelial cells suggested that Class I pili may mediate bacterial adherence with all three epithelial cell lines. In contrast, Class II pili of the strains used did not increase bacterial adherence to Hep-2 larynx carcinoma cells, although an increase in adherence to Chang conjunctival and A549 lung carcinoma epithelial cells was observed in the Class II pili-expressing strains. In addition to these interclass functional variations, differences in adherence to epithelial cells were also observed among Class I and Class II strains. Functionally different pilin variants of one Class I strain, MC58, were obtained by single colony isolation. One piliated variant was identified which had concurrently lost the ability to adhere to both Chang and Hep-2 cells ('non-adherent' phenotype; adherence of less than 2 bacteria per cell). In addition, several adherent pilin variants were isolated from non-adherent Pil- and Pil+ bacteria by selection on Chang cells (adherence of 10-25 bacteria per cell). In contrast to epithelial cells, all variant pili, whether of Class I or Class II, adhered to endothelial cells in substantially larger numbers (greater than 50 bacteria per cell) and therefore implied the existence of distinct mechanisms in pilus-facilitated interactions of N. meningitidis with endothelial and epithelial cells.
|
pubmed:grant | |
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
May
|
pubmed:issn |
0950-382X
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
6
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
1271-9
|
pubmed:dateRevised |
2009-9-29
|
pubmed:meshHeading |
pubmed-meshheading:1353602-Bacterial Adhesion,
pubmed-meshheading:1353602-Bacterial Outer Membrane Proteins,
pubmed-meshheading:1353602-Cells, Cultured,
pubmed-meshheading:1353602-Endothelium, Vascular,
pubmed-meshheading:1353602-Epithelial Cells,
pubmed-meshheading:1353602-Epithelium,
pubmed-meshheading:1353602-Fimbriae, Bacterial,
pubmed-meshheading:1353602-Fimbriae Proteins,
pubmed-meshheading:1353602-Humans,
pubmed-meshheading:1353602-Neisseria meningitidis,
pubmed-meshheading:1353602-Phenotype,
pubmed-meshheading:1353602-Species Specificity,
pubmed-meshheading:1353602-Tumor Cells, Cultured,
pubmed-meshheading:1353602-Umbilical Veins
|
pubmed:year |
1992
|
pubmed:articleTitle |
Variations in the expression of pili: the effect on adherence of Neisseria meningitidis to human epithelial and endothelial cells.
|
pubmed:affiliation |
Department of Paediatrics, University of Oxford, John Radcliffe Hospital, UK.
|
pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|