|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
5052
|
|
pubmed:dateCreated |
1992-4-30
|
|
pubmed:abstractText |
Guanosine triphosphate (GTP)-binding proteins are required for intracellular vesicular transport. Mastoparan is a peptide component of wasp venom that increases nucleotide exchange in some classes of G alpha subunits of regulatory heterotrimeric GTP-binding proteins (G proteins). Mastoparan and other compounds that increase nucleotide exchange by G proteins inhibited endosome fusion in vitro and reversed the effects of guanosine 5'-O-(3-thiotriphosphate) (GTP-gamma-S), a nonhydrolyzable GTP analog. Addition of beta gamma subunits of G proteins to the fusion assay antagonized the stimulatory effect of GTP-gamma-S, confirming the participation of G proteins. These results indicate that GTP-binding proteins are required for endosome fusion and in particular that a G protein is involved. Given the function of G proteins in signal transduction, these findings may provide insight into the mechanism by which endosomal vesicles become competent for fusion after their formation at the cell surface.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Mar
|
|
pubmed:issn |
0036-8075
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
27
|
|
pubmed:volume |
255
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
1695-7
|
|
pubmed:dateRevised |
2007-11-14
|
|
pubmed:meshHeading |
pubmed-meshheading:1348148-Biological Transport,
pubmed-meshheading:1348148-Endocytosis,
pubmed-meshheading:1348148-Endosomes,
pubmed-meshheading:1348148-GTP-Binding Proteins,
pubmed-meshheading:1348148-Guanosine 5'-O-(3-Thiotriphosphate),
pubmed-meshheading:1348148-Guanosine Diphosphate,
pubmed-meshheading:1348148-Guanosine Triphosphate,
pubmed-meshheading:1348148-Intracellular Membranes,
pubmed-meshheading:1348148-Macromolecular Substances,
pubmed-meshheading:1348148-Membrane Fusion,
pubmed-meshheading:1348148-Organelles,
pubmed-meshheading:1348148-Peptides,
pubmed-meshheading:1348148-Wasp Venoms
|
|
pubmed:year |
1992
|
|
pubmed:articleTitle |
Evidence of a role for heterotrimeric GTP-binding proteins in endosome fusion.
|
|
pubmed:affiliation |
Department of Cell Biology and Physiology, Washington University, School of Medicine, St. Louis, MO 63110.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|
