Linked Life Data

1326521

Source:http://linkedlifedata.com/resource/pubmed/id/1326521

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
26
pubmed:dateCreated
1992-10-19
pubmed:abstractText
In 1989, Shier and Watt identified a gene which was predicted to encode a new member of the insulin receptor (IR) family, and they called it the insulin receptor-related receptor (IRR) (Shier, P., and Watt, V. M. (1989) J. Biol. Chem. 264, 14605-14608). However, the tissues expressing this receptor, its ligand binding specificity and its signaling capability have remained unknown. In the present studies we report Northern blot analyses and polymerase chain reaction data, which indicate that the IRR mRNA is expressed in a variety of tissues, including the human kidney, heart, skeletal muscle, liver, and pancreas. In order to examine the ligand(s) recognized by IRR, we constructed a chimeric receptor with the extracellular domain of the IR replaced with that of IRR. This chimera was found not to bind radioactively labeled insulin, insulin-like growth factor I (IGF-I), or IGF-II. These ligands and relaxin, the only other known member of the mammalian insulin family, also failed to stimulate the tyrosine kinase activity of this chimeric receptor. A second chimeric receptor with the extracellular domain of IR and the kinase domain of IRR was also constructed and utilized to study the signaling capabilities of the kinase domain of IRR. This chimera exhibited high affinity insulin binding and insulin-stimulated tyrosine kinase activity. The kinase domains of the IR and IRR were found capable of phosphorylating the same spectrum of exogenous and endogenous substrates. However, Chinese hamster ovary (CHO) cells stably overexpressing the kinase domain of IRR exhibited elevated basal thymidine incorporation and 2-deoxyglucose uptake compared with CHO cells and CHO cells overexpressing wild-type IR. We conclude that: 1) IRR is expressed in the human kidney, heart, skeletal muscle, liver, and pancreas, 2) IRR does not appear to be the receptor of any known member of the insulin family, and 3) the tyrosine kinase of IRR appears to be similar to that of IR in both the spectrum of substrates phosphorylated and the biological responses stimulated.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
267
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
18320-8
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:1326521-1-Phosphatidylinositol 4-Kinase, pubmed-meshheading:1326521-Animals, pubmed-meshheading:1326521-Base Sequence, pubmed-meshheading:1326521-Blotting, Northern, pubmed-meshheading:1326521-Blotting, Western, pubmed-meshheading:1326521-CHO Cells, pubmed-meshheading:1326521-Cells, Cultured, pubmed-meshheading:1326521-Cricetinae, pubmed-meshheading:1326521-DNA, pubmed-meshheading:1326521-Deoxyglucose, pubmed-meshheading:1326521-GTPase-Activating Proteins, pubmed-meshheading:1326521-Insulin, pubmed-meshheading:1326521-Molecular Sequence Data, pubmed-meshheading:1326521-Phosphorylation, pubmed-meshheading:1326521-Phosphotransferases, pubmed-meshheading:1326521-Polymerase Chain Reaction, pubmed-meshheading:1326521-Precipitin Tests, pubmed-meshheading:1326521-Proteins, pubmed-meshheading:1326521-Receptor, Insulin, pubmed-meshheading:1326521-Signal Transduction, pubmed-meshheading:1326521-Substrate Specificity, pubmed-meshheading:1326521-Thymidine, pubmed-meshheading:1326521-Tissue Distribution, pubmed-meshheading:1326521-Tyrosine
pubmed:year
1992
pubmed:articleTitle
The insulin receptor-related receptor. Tissue expression, ligand binding specificity, and signaling capabilities.
pubmed:affiliation
Department of Pharmacology, Stanford University School of Medicine, California 94305.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't