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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1992-9-10
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pubmed:abstractText |
The ability of vasculitis-associated anti-neutrophil cytoplasm antibodies (ANCA) to activate neutrophils and mediate release of radiolabel from 111Indium-labeled cultured human umbilical vein endothelial cells (HUVEC) was determined as a measure of the potential cytotoxicity of ANCA-activated neutrophils against vascular endothelium. Priming of neutrophils with low doses of phorbol 12-myristate 13-acetate (PMA) (1 ng/ml) and ionomycin (0.1 mumol/1) was required, together with pretreatment of endothelial cells with BCNU (1,3-bis-[2-chloroethyl]-1-nitrosourea; 0.26 mmol/l). Under these conditions and using a 4-hour serum-free assay system, mouse monoclonal antibodies (MAb) to the target autoantigens proteinase-3 (Pr-3) and myeloperoxidase (MPO) mediated enhanced release of 111Indium from HUVEC compared with control MAb. Human IgG Fab2 C-ANCA (recognizing Pr-3) and P-ANCA (recognizing MPO) did likewise. Preactivation of HUVEC with TNF (50 U/ml, 4 hr) enhanced the release of 111Indium from HUVEC generated by neutrophils activated with anti-Pr-3 and anti-MPO MAb. These data support the suggestion that activation of neutrophils by ANCA within the vascular lumen may contribute to endothelial cell injury.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1323218-12412739,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1323218-1658170,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1323218-1690903,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1323218-1694204,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1323218-1696867,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1323218-1754104,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1323218-1974032,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1323218-1987304,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1323218-1987472,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1323218-2070557,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1323218-2161532,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1323218-2181020,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1323218-2193079,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1323218-2357853,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1323218-2453802,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1323218-2475511,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1323218-2536474,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1323218-2657720,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1323218-2660645,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1323218-2747218,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1323218-2984939,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1323218-3030114,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1323218-3261375,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1323218-3485659,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1323218-4355998,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1323218-6635659,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1323218-6707200
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Antineutrophil...,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Autoantibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Carmustine,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Fab Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Ionomycin,
http://linkedlifedata.com/resource/pubmed/chemical/Myeloblastin,
http://linkedlifedata.com/resource/pubmed/chemical/Peroxidase,
http://linkedlifedata.com/resource/pubmed/chemical/Serine Endopeptidases,
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0002-9440
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
141
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
335-42
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pubmed:dateRevised |
2010-9-7
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pubmed:meshHeading |
pubmed-meshheading:1323218-Antibodies, Antineutrophil Cytoplasmic,
pubmed-meshheading:1323218-Antibodies, Monoclonal,
pubmed-meshheading:1323218-Antibody Formation,
pubmed-meshheading:1323218-Autoantibodies,
pubmed-meshheading:1323218-Carmustine,
pubmed-meshheading:1323218-Cytotoxicity, Immunologic,
pubmed-meshheading:1323218-Endothelium, Vascular,
pubmed-meshheading:1323218-Humans,
pubmed-meshheading:1323218-Immunoglobulin Fab Fragments,
pubmed-meshheading:1323218-Ionomycin,
pubmed-meshheading:1323218-Myeloblastin,
pubmed-meshheading:1323218-Neutrophils,
pubmed-meshheading:1323218-Peroxidase,
pubmed-meshheading:1323218-Serine Endopeptidases,
pubmed-meshheading:1323218-Tetradecanoylphorbol Acetate,
pubmed-meshheading:1323218-Tumor Necrosis Factor-alpha,
pubmed-meshheading:1323218-Vasculitis
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pubmed:year |
1992
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pubmed:articleTitle |
Autoantibodies developing to myeloperoxidase and proteinase 3 in systemic vasculitis stimulate neutrophil cytotoxicity toward cultured endothelial cells.
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pubmed:affiliation |
Section of Vascular Biology, Royal Postgraduate Medical School, London, United Kingdom.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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