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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5070
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pubmed:dateCreated |
1992-9-4
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pubmed:abstractText |
The Wilms tumor suppressor gene wt1 encodes a zinc finger DNA binding protein, WT1, that functions as a transcriptional repressor. The fetal mitogen insulin-like growth factor II (IGF-II) is overexpressed in Wilms tumors and may have autocrine effects in tumor progression. The major fetal IGF-II promoter was defined in transient transfection assays as a region spanning from nucleotides -295 to +135, relative to the transcription start site. WT1 bound to multiple sites in this region and functioned as a potent repressor of IGF-II transcription in vivo. Maximal repression was dependent on the presence of WT1 binding sites on each side of the transcriptional initiation site. These findings provide a molecular basis for overexpression of IGF-II in Wilms tumors and suggest that WT1 negatively regulates blastemal cell proliferation by limiting the production of a fetal growth factor in the developing vertebrate kidney.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0036-8075
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
31
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pubmed:volume |
257
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
674-8
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:1323141-Animals,
pubmed-meshheading:1323141-Base Sequence,
pubmed-meshheading:1323141-Binding Sites,
pubmed-meshheading:1323141-Blotting, Northern,
pubmed-meshheading:1323141-DNA,
pubmed-meshheading:1323141-DNA-Binding Proteins,
pubmed-meshheading:1323141-Deoxyribonuclease I,
pubmed-meshheading:1323141-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:1323141-Genes, Wilms Tumor,
pubmed-meshheading:1323141-Humans,
pubmed-meshheading:1323141-Insulin-Like Growth Factor II,
pubmed-meshheading:1323141-Kidney,
pubmed-meshheading:1323141-Mice,
pubmed-meshheading:1323141-Molecular Sequence Data,
pubmed-meshheading:1323141-Promoter Regions, Genetic,
pubmed-meshheading:1323141-Rats,
pubmed-meshheading:1323141-Sequence Homology, Nucleic Acid,
pubmed-meshheading:1323141-Transfection,
pubmed-meshheading:1323141-WT1 Proteins,
pubmed-meshheading:1323141-Wilms Tumor,
pubmed-meshheading:1323141-Zinc Fingers
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pubmed:year |
1992
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pubmed:articleTitle |
Repression of the insulin-like growth factor II gene by the Wilms tumor suppressor WT1.
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pubmed:affiliation |
Howard Hughes Medical Institute, University of Chicago, IL 60637.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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