Linked Life Data

1321211

Source:http://linkedlifedata.com/resource/pubmed/id/1321211

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1992-8-18
pubmed:abstractText
HeLa cells were preincubated with radiolabelled poliovirus type 1 at 26 degrees C, such that the 160S virions were internalized, but not altered structurally. The temperature was then shifted to 37 degrees C to study the intracellular redistribution of the virions and the modifications they undergo at that temperature. Using subcellular fractionation in isoosmotic Nycodenz gradients, we obtained evidence for the rapid loss of virions from the plasma membrane and from a vesicular fraction, as well as for the formation of two populations of intracellular 135S particles. The first population was associated with lysosomes and was slowly converted to (RNA-containing) 110S particles. In the presence of the lysosomotropic agent chloroquine, the lysosomal 135S population was converted to 80S empty capsids. The second 135S population, which was not associated with any organelle, was converted to 80S empty capsids. Similar observations were made during unsynchronized infection at 37 degrees C. We propose a model for infection in which 135S particles cross a membrane barrier, and are uncoated in the cytosol.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0022-1317
pubmed:author
pubmed:issnType
Print
pubmed:volume
73 ( Pt 7)
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1739-44
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Compartmentalization of subviral particles during poliovirus eclipse in HeLa cells.
pubmed:affiliation
Department of Microbiology and Hygiene, Vrije Universiteit Brussel, Belgium.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't