Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1992-7-28
pubmed:abstractText
Expression of the mouse beta-PDGF receptor by gene transfer confers PDGF-dependent and reversible neuronal differentiation of PC12 pheochromocytoma cells similar to that observed in response to NGF and basic FGF. A common property of the PDGF, NGF, and basic FGF-induced differentiation response is the requirement for constant exposure of cells to the growth factor. To test the hypothesis that a persistent level of growth factor receptor signaling is required for the maintenance of the neuronal phenotype, we examined the regulation of the serine/threonine-specific MAP kinases after either short- (10 min) or long-term (24 h) stimulation with growth factors. Mono Q FPLC resolved two peaks of growth factor-stimulated MAP kinase activity that coeluted with tyrosine phosphorylated 41- and 43-kDa polypeptides. MAP kinase activity was markedly stimulated (approximately 30-fold) within 5 min of exposure to several growth factors (PDGF, NGF, basic FGF, EGF, and IGF-I), but was persistently maintained at 10-fold above basal activity after 24 h only by the growth factors that also induce PC12 cell differentiation (PDGF, NGF, and basic FGF). Thus the beta-PDGF receptor is in a subset of tyrosine kinase-encoded growth factor receptors that are capable of maintaining continuous signals required for differentiation of PC12 cells. These signals include the constitutive activation of cytoplasmic serine/threonine protein kinases.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1319243-1065897, http://linkedlifedata.com/resource/pubmed/commentcorrection/1319243-1647176, http://linkedlifedata.com/resource/pubmed/commentcorrection/1319243-1653029, http://linkedlifedata.com/resource/pubmed/commentcorrection/1319243-1657919, http://linkedlifedata.com/resource/pubmed/commentcorrection/1319243-1700979, http://linkedlifedata.com/resource/pubmed/commentcorrection/1319243-1705548, http://linkedlifedata.com/resource/pubmed/commentcorrection/1319243-1705885, http://linkedlifedata.com/resource/pubmed/commentcorrection/1319243-1706478, http://linkedlifedata.com/resource/pubmed/commentcorrection/1319243-1801927, http://linkedlifedata.com/resource/pubmed/commentcorrection/1319243-1832020, http://linkedlifedata.com/resource/pubmed/commentcorrection/1319243-1848672, http://linkedlifedata.com/resource/pubmed/commentcorrection/1319243-1848706, http://linkedlifedata.com/resource/pubmed/commentcorrection/1319243-1849075, http://linkedlifedata.com/resource/pubmed/commentcorrection/1319243-1849270, http://linkedlifedata.com/resource/pubmed/commentcorrection/1319243-1849459, http://linkedlifedata.com/resource/pubmed/commentcorrection/1319243-1850551, http://linkedlifedata.com/resource/pubmed/commentcorrection/1319243-1850821, http://linkedlifedata.com/resource/pubmed/commentcorrection/1319243-2032290, http://linkedlifedata.com/resource/pubmed/commentcorrection/1319243-2142153, http://linkedlifedata.com/resource/pubmed/commentcorrection/1319243-2154696, http://linkedlifedata.com/resource/pubmed/commentcorrection/1319243-2155237, http://linkedlifedata.com/resource/pubmed/commentcorrection/1319243-2158859, http://linkedlifedata.com/resource/pubmed/commentcorrection/1319243-2171996, http://linkedlifedata.com/resource/pubmed/commentcorrection/1319243-2174361, http://linkedlifedata.com/resource/pubmed/commentcorrection/1319243-2394735, http://linkedlifedata.com/resource/pubmed/commentcorrection/1319243-2406028, http://linkedlifedata.com/resource/pubmed/commentcorrection/1319243-2457918, http://linkedlifedata.com/resource/pubmed/commentcorrection/1319243-2473471, http://linkedlifedata.com/resource/pubmed/commentcorrection/1319243-2544996, http://linkedlifedata.com/resource/pubmed/commentcorrection/1319243-2549192, http://linkedlifedata.com/resource/pubmed/commentcorrection/1319243-2555160, http://linkedlifedata.com/resource/pubmed/commentcorrection/1319243-2631796, http://linkedlifedata.com/resource/pubmed/commentcorrection/1319243-2673544, http://linkedlifedata.com/resource/pubmed/commentcorrection/1319243-2784535, http://linkedlifedata.com/resource/pubmed/commentcorrection/1319243-2785993, http://linkedlifedata.com/resource/pubmed/commentcorrection/1319243-2831375, http://linkedlifedata.com/resource/pubmed/commentcorrection/1319243-2836147, http://linkedlifedata.com/resource/pubmed/commentcorrection/1319243-2838784, http://linkedlifedata.com/resource/pubmed/commentcorrection/1319243-2993898, http://linkedlifedata.com/resource/pubmed/commentcorrection/1319243-3282875, http://linkedlifedata.com/resource/pubmed/commentcorrection/1319243-3316527, http://linkedlifedata.com/resource/pubmed/commentcorrection/1319243-6259177, http://linkedlifedata.com/resource/pubmed/commentcorrection/1319243-999827
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1059-1524
pubmed:author
pubmed:issnType
Print
pubmed:volume
3
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
545-53
pubmed:dateRevised
2010-9-7
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
The beta-PDGF receptor induces neuronal differentiation of PC12 cells.
pubmed:affiliation
Division of Basic Sciences, National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado 80206.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't