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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
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pubmed:dateCreated |
1992-5-5
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pubmed:abstractText |
A cell line (SCMC-MM-1) was established from a human abdominal tumor that was initially diagnosed as a malignant mesenchymoma by histological, immunohistochemical and clinical criteria. The cell line was composed of 2 morphologically and immunohistochemically distinct cell types, one with a small polygonal phenotype (P-type), characterized by the immunostaining of vimentin and the presence of a few electron-microscopically visible organelles, and the other with a giant tubular phenotype (T-type), characterized by the immunostaining of desmin, alpha-sarcomeric actin and skeletal-muscle myosin, and the presence of thick and thin myofilaments and Z-line materials. The parental cell line was cloned into 2 sublines, a P-type clone (SCMC-MM-1-19P) and a T-type clone (SCMC-MM-1-1T), which shared both 2q37 and 11p15 translocations, the characteristic chromosomal aberrations for rhabdomyosarcoma, with the parental SCMC-MM-1 cell line. Northern-blot analyses of the myogenic regulatory genes, including MyoD1 and myogenin, demonstrated the expression of MyoD1 in both of these sublines. Myogenin was very weakly expressed in the SCMC-MM-1-19P subline, but strongly expressed in the SCMC-MM-1-1T subline. Chromosomal and myogenic-regulatory-gene analyses revealed that both of these sublines were rhabdomyosarcoma cell lines. Furthermore, the regulatory-gene analyses indicated that these 2 sublines represented 2 distinct differentiation stages of myoblasts, and that MyoD1 and myogenin could serve as the lineage marker and the differentiation marker, respectively, of human rhabdomyosarcoma.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Genetic Markers,
http://linkedlifedata.com/resource/pubmed/chemical/MYOG protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Muscle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/MyoD Protein,
http://linkedlifedata.com/resource/pubmed/chemical/MyoD1 myogenic differentiation...,
http://linkedlifedata.com/resource/pubmed/chemical/Myogenin,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0020-7136
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
50
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
977-83
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pubmed:dateRevised |
2007-7-24
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pubmed:meshHeading |
pubmed-meshheading:1313401-Blotting, Northern,
pubmed-meshheading:1313401-Chromosome Banding,
pubmed-meshheading:1313401-Clone Cells,
pubmed-meshheading:1313401-Fluorescent Antibody Technique,
pubmed-meshheading:1313401-Genes, Regulator,
pubmed-meshheading:1313401-Genetic Markers,
pubmed-meshheading:1313401-Humans,
pubmed-meshheading:1313401-Karyotyping,
pubmed-meshheading:1313401-Microscopy, Electron,
pubmed-meshheading:1313401-Muscle Proteins,
pubmed-meshheading:1313401-MyoD Protein,
pubmed-meshheading:1313401-Myogenin,
pubmed-meshheading:1313401-Nuclear Proteins,
pubmed-meshheading:1313401-Phosphoproteins,
pubmed-meshheading:1313401-RNA, Messenger,
pubmed-meshheading:1313401-Rhabdomyosarcoma,
pubmed-meshheading:1313401-Tumor Cells, Cultured
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pubmed:year |
1992
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pubmed:articleTitle |
Differential expression of myogenic regulatory genes, MyoD1 and myogenin, in human rhabdomyosarcoma sublines.
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pubmed:affiliation |
Department of Pediatrics, Kyoto Prefectural University of Medicine, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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