|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
1993-6-24
|
|
pubmed:abstractText |
Sense mRNA coding for bovine adrenal medulla aromatic L-amino acid decarboxylase (AADC) was expressed following microinjection into Xenopus laevis oocytes. The expressed enzyme activity was stereoselective for L-5-hydroxytryptophan and L-DOPA and blocked by NSD-1015 an inhibitor of AADC. Heating the expressed enzyme at 55 degrees C resulted in a parallel loss of activity towards both substrates. Our findings are consistent with the prevailing notion that a single enzyme is able to decarboxylate both substrates in vivo.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Sep
|
|
pubmed:issn |
0197-0186
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
21
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
275-9
|
|
pubmed:dateRevised |
2007-11-14
|
|
pubmed:meshHeading |
pubmed-meshheading:1303156-Adrenal Glands,
pubmed-meshheading:1303156-Animals,
pubmed-meshheading:1303156-Aromatic-L-Amino-Acid Decarboxylases,
pubmed-meshheading:1303156-Cattle,
pubmed-meshheading:1303156-Cloning, Molecular,
pubmed-meshheading:1303156-DNA,
pubmed-meshheading:1303156-Female,
pubmed-meshheading:1303156-Gene Library,
pubmed-meshheading:1303156-Kinetics,
pubmed-meshheading:1303156-Microinjections,
pubmed-meshheading:1303156-Oocytes,
pubmed-meshheading:1303156-Protein Biosynthesis,
pubmed-meshheading:1303156-RNA, Messenger,
pubmed-meshheading:1303156-Transcription, Genetic,
pubmed-meshheading:1303156-Xenopus laevis
|
|
pubmed:year |
1992
|
|
pubmed:articleTitle |
Expression of cloned aromatic L-amino acid decarboxylase in Xenopus laevis oocytes.
|
|
pubmed:affiliation |
Department of Pharmacology, Ohio State University College of Medicine, Columbus 43210.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|
