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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
2003-9-29
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pubmed:abstractText |
Caspases are important for apoptosis but are also involved in mammalian cell survival and cell division. Here we report that caspase-3 is a negative regulator of B cell cycling. Mice deficient in caspase-3 (Casp3-/- mice) have increased numbers of splenic B cells that show normal apoptosis but enhanced proliferation in vivo and hyperproliferation after mitogenic stimulation in vitro. Cdkn1a encodes p21 (also called Waf1 or Cip1), a cyclin-dependent kinase (CDK) inhibitor. Although expression of p21 was increased, CDK activities and proliferating cell nuclear antigen (PCNA) were increased in Casp3-/- B cells. Using Casp3-/-Cdkn1a-/- mice, we show that the hyperproliferation of Casp3-/- B cells is abolished when Cdkn1a is also deleted. Our genetic and biochemical data demonstrate that caspase-3 is essential in the regulation of B cell homeostasis.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bromodeoxyuridine,
http://linkedlifedata.com/resource/pubmed/chemical/Casp3 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Caspase 3,
http://linkedlifedata.com/resource/pubmed/chemical/Caspases,
http://linkedlifedata.com/resource/pubmed/chemical/Cdkn1a protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinase Inhibitor...,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclins,
http://linkedlifedata.com/resource/pubmed/chemical/Proliferating Cell Nuclear Antigen
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1529-2908
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pubmed:author |
pubmed-author:BouchardDenisD,
pubmed-author:DuncanGordon SGS,
pubmed-author:FurlongerCarenC,
pubmed-author:HakemAnneA,
pubmed-author:HakemRazqallahR,
pubmed-author:LuLiweiL,
pubmed-author:MakTak WTW,
pubmed-author:PaigeChristopher JCJ,
pubmed-author:SasakiTakehikoT,
pubmed-author:WooMinnaM,
pubmed-author:WuGillian EGE
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pubmed:issnType |
Print
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pubmed:volume |
4
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1016-22
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:12970760-Animals,
pubmed-meshheading:12970760-Apoptosis,
pubmed-meshheading:12970760-B-Lymphocytes,
pubmed-meshheading:12970760-Bromodeoxyuridine,
pubmed-meshheading:12970760-Caspase 3,
pubmed-meshheading:12970760-Caspases,
pubmed-meshheading:12970760-Cell Cycle,
pubmed-meshheading:12970760-Cyclin-Dependent Kinase Inhibitor p21,
pubmed-meshheading:12970760-Cyclin-Dependent Kinases,
pubmed-meshheading:12970760-Cyclins,
pubmed-meshheading:12970760-Flow Cytometry,
pubmed-meshheading:12970760-Immunohistochemistry,
pubmed-meshheading:12970760-Lymphocyte Activation,
pubmed-meshheading:12970760-Mice,
pubmed-meshheading:12970760-Mice, Inbred C57BL,
pubmed-meshheading:12970760-Mice, Knockout,
pubmed-meshheading:12970760-Proliferating Cell Nuclear Antigen,
pubmed-meshheading:12970760-Spleen,
pubmed-meshheading:12970760-Substrate Specificity
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pubmed:year |
2003
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pubmed:articleTitle |
Caspase-3 regulates cell cycle in B cells: a consequence of substrate specificity.
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pubmed:affiliation |
Ontario Cancer Institute, Toronto, Ontario M5G 2N9, Canada.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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