Source:http://linkedlifedata.com/resource/pubmed/id/12964046
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
2003-9-9
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pubmed:abstractText |
We studied the role of IL-2, IL-15, IL-10, TNF and IL-2 receptor complexes (IL-2R) produced constitutively by a T-cell lymphoma line (LBC) on their own proliferation. The constitutive expression of surface alpha, beta and gamma chains IL-2R was detected in tumor cells by flow cytometry. Using reverse-transcription PCR, mRNA for IL-2, IL-15, IL-10 and TNF were found to be present in LBC. In addition, tumor cells were found to constitutively express intracellular IL-2, IL-15, IL-10 and TNF. Despite the production of these cytokines by tumor cells, specific neutralising antibodies did not inhibit LBC proliferation; surprisingly, anti-IL-15 increased LBC cell growth. We also demonstrated that recombinant IL-2 or IL-15 enhanced LBC cell proliferation. Our data suggest that endogenous IL-2 and IL-15 may trigger the proliferation of lymphoma LBC cells, and so their growth could be regulated, at least partly, by IL-2/IL-15/IL-2R system. In addition, IL-10 and TNF, immunosuppressor and pro-metastatic cytokines, respectively, may promote the in vivo growth of the tumor. The fact that leukaemia-lymphoma cells produce simultaneously both IL-2 and IL-15 should be taken into consideration in the design of immunotherapy protocols directed to IL-2R.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-10,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-15,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1107-3756
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
12
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
627-32
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12964046-Animals,
pubmed-meshheading:12964046-Cell Division,
pubmed-meshheading:12964046-Cell Line, Tumor,
pubmed-meshheading:12964046-Cytokines,
pubmed-meshheading:12964046-Flow Cytometry,
pubmed-meshheading:12964046-Interleukin-10,
pubmed-meshheading:12964046-Interleukin-15,
pubmed-meshheading:12964046-Interleukin-2,
pubmed-meshheading:12964046-Lymphoma, T-Cell,
pubmed-meshheading:12964046-Mice,
pubmed-meshheading:12964046-RNA, Messenger,
pubmed-meshheading:12964046-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:12964046-Time Factors,
pubmed-meshheading:12964046-Tumor Necrosis Factor-alpha
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pubmed:year |
2003
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pubmed:articleTitle |
IL-2, IL-10, IL-15 and TNF are key regulators of murine T-cell lymphoma growth.
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pubmed:affiliation |
Centro de Estudios Farmacológicos y Botánicos (CEFYBO), CONICET, Serrano 669, Buenos Aires 1414, Argentina. majo@interserver.com.ar
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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