Source:http://linkedlifedata.com/resource/pubmed/id/12963271
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3-4
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pubmed:dateCreated |
2003-9-9
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pubmed:abstractText |
We established an enzyme-linked immunosorbent assay (ELISA) system for the quantitation of bovine macrophage colony-stimulating factor (M-CSF) and used it to measure the serum M-CSF levels in bovine fetuses and calves. The average serum M-CSF level was 2.7+/-1.5 ng/ml in 39 calves under 100 days old, and 1.8+/-0.8 ng/ml in 15 cattle between 101 and 418 days old. Fetal sera samples (n = 6) prepared from cattle between 150 and 280 days of gestational age had a higher average level of M-CSF (8.8+/-1.4 ng/ml). Alteration in serum M-CSF levels in each individual calf was also measured. The serum levels of M-CSF in calves at 0-1 day after birth ranged from 0.52 to 7.3 ng/ml. During the period 113-125 days after birth, serum levels were around 1.4+/-0.39 ng/ml. Although serum M-CSF levels generally decreased as the age of calves advanced, differences among individuals, especially among newborn calves, were observed.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0165-2427
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pubmed:author |
pubmed-author:HiraiTT,
pubmed-author:InumaruSS,
pubmed-author:KikumaRR,
pubmed-author:KikyoYY,
pubmed-author:KubotaCC,
pubmed-author:MatsuuraSS,
pubmed-author:MoriYY,
pubmed-author:MunetaYY,
pubmed-author:OshimaKK,
pubmed-author:SatohMM,
pubmed-author:SatohNN,
pubmed-author:YayotaCC,
pubmed-author:YokomizoYY,
pubmed-author:YoshiharaKK
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
95
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
103-11
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12963271-Age Factors,
pubmed-meshheading:12963271-Animals,
pubmed-meshheading:12963271-Animals, Newborn,
pubmed-meshheading:12963271-Blotting, Western,
pubmed-meshheading:12963271-Cattle,
pubmed-meshheading:12963271-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:12963271-Female,
pubmed-meshheading:12963271-Fetus,
pubmed-meshheading:12963271-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:12963271-Pregnancy,
pubmed-meshheading:12963271-Sensitivity and Specificity
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pubmed:year |
2003
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pubmed:articleTitle |
Quantitation of bovine macrophage colony-stimulating factor in bovine serum by ELISA.
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pubmed:affiliation |
National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan. kazukazu@affrc.go.jp
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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