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rdf:type |
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lifeskim:mentions |
umls-concept:C0014597,
umls-concept:C0031727,
umls-concept:C0086418,
umls-concept:C0205263,
umls-concept:C0205282,
umls-concept:C0227843,
umls-concept:C0332120,
umls-concept:C0387583,
umls-concept:C0597716,
umls-concept:C0851285,
umls-concept:C1314939,
umls-concept:C1516859
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pubmed:issue |
1
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pubmed:dateCreated |
2003-8-13
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pubmed:abstractText |
We previously reported that human malignant endometrial epithelial cell conditioned medium (MECM) up-regulated cyclooxygenase (COX)-2 mRNA and protein levels in human normal endometrial stromal cells (ESC). Here we showed that pretreatment with a selective inhibitor of the extracellularly regulated kinase (ERK)1/2 signaling pathway blocked the MECM-induced COX-2 expression in ESC. Transient transfection assays indicated critical roles of a cAMP response element (CRE,-59/-53 bp) and a nuclear factor for interleukin (IL)-6 expression (NF-IL6) site (-132/-124 bp) in the regulation of basal and MECM-induced activity of COX-2 gene promoter in ESC. Employing electrophoretic mobility shift assays, we demonstrated that increased functional binding of CCAAT/enhancer binding protein (C/EBP)alpha, C/EBPbeta and upstream stimulatory factor-2 to the CRE and C/EBPalpha and C/EBPbeta to the NF-IL6 site were, at least in part, responsible for MECM-induced COX-2 expression in ESC. Moreover, overexpression of C/EBPalpha and C/EBPbeta significantly induced COX-2 promoter activity in ESC. Collectively, these results suggest that the basal and MECM-induced transcription of the COX-2 gene in ESC is regulated through a combination of the CRE and the NF-IL6 site by functional interactions of C/EBPalpha and C/EBPbeta.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CCAAT-Enhancer-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Conditioned,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase 2,
http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotide Probes,
http://linkedlifedata.com/resource/pubmed/chemical/PTGS2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Prostaglandin-Endoperoxide Synthases
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0952-5041
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
31
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
95-104
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:12914528-Base Sequence,
pubmed-meshheading:12914528-CCAAT-Enhancer-Binding Proteins,
pubmed-meshheading:12914528-Culture Media, Conditioned,
pubmed-meshheading:12914528-Cyclooxygenase 2,
pubmed-meshheading:12914528-Endometrial Neoplasms,
pubmed-meshheading:12914528-Enzyme Induction,
pubmed-meshheading:12914528-Female,
pubmed-meshheading:12914528-Humans,
pubmed-meshheading:12914528-Isoenzymes,
pubmed-meshheading:12914528-Membrane Proteins,
pubmed-meshheading:12914528-Mitogen-Activated Protein Kinases,
pubmed-meshheading:12914528-Mutagenesis,
pubmed-meshheading:12914528-Oligonucleotide Probes,
pubmed-meshheading:12914528-Prostaglandin-Endoperoxide Synthases,
pubmed-meshheading:12914528-Restriction Mapping,
pubmed-meshheading:12914528-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:12914528-Sequence Deletion,
pubmed-meshheading:12914528-Stromal Cells,
pubmed-meshheading:12914528-Transfection,
pubmed-meshheading:12914528-Tumor Cells, Cultured
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pubmed:year |
2003
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pubmed:articleTitle |
Induction of cyclooxygenase-2 in human endometrial stromal cells by malignant endometrial epithelial cells: evidence for the involvement of extracellularly regulated kinases and CCAAT/enhancer binding proteins.
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pubmed:affiliation |
Departments of Obstetrics and Gynecology, The University of Illinois at Chicago, Chicago, Illinois 60612, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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