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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2003-7-21
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pubmed:abstractText |
Immune responses to the factor IX protein pose problems for hemophilia B patients who develop antibodies against factor IX and for potential future treatment with gene therapy. To better define the response to human factor IX, we analyzed T-cell responses to human factor IX in factor IX knockout mice on BALB/c and C57BL/6 (B6) backgrounds, both strains having CD4+ T cells that proliferate in response to human factor IX. Surprisingly, wild-type mice have similar factor IX-recognizing CD4+ T cells. We defined a dominant CD4+ epitope for each strain (CVETGVKITVVAGEH for BALB/c and LLELDEPLVLNSYVTPIC for B6) that was recognized by both factor IX knockout and wild-type mice. While human factor IX did not cross-react with the mouse homologs of these epitopes, immunization with peptides from murine factor IX stimulated proliferation in factor IX knockout mice and wild-type mice, demonstrating a failure to delete murine factor IX-specific T cells in normal mice.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1538-7933
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
1
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
95-102
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pubmed:dateRevised |
2006-11-7
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pubmed:meshHeading |
pubmed-meshheading:12871545-Amino Acid Sequence,
pubmed-meshheading:12871545-Animals,
pubmed-meshheading:12871545-CD4-Positive T-Lymphocytes,
pubmed-meshheading:12871545-Cross Reactions,
pubmed-meshheading:12871545-Epitopes, T-Lymphocyte,
pubmed-meshheading:12871545-Factor IX,
pubmed-meshheading:12871545-Humans,
pubmed-meshheading:12871545-Immune Tolerance,
pubmed-meshheading:12871545-Immunization,
pubmed-meshheading:12871545-Immunoglobulin G,
pubmed-meshheading:12871545-Lymphocyte Activation,
pubmed-meshheading:12871545-Mice,
pubmed-meshheading:12871545-Mice, Inbred BALB C,
pubmed-meshheading:12871545-Mice, Inbred C57BL,
pubmed-meshheading:12871545-Mice, Knockout,
pubmed-meshheading:12871545-Molecular Sequence Data,
pubmed-meshheading:12871545-Peptide Fragments,
pubmed-meshheading:12871545-Recombinant Proteins,
pubmed-meshheading:12871545-Sequence Homology, Amino Acid
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pubmed:year |
2003
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pubmed:articleTitle |
Identification of T-cell epitopes in clotting factor IX and lack of tolerance in inbred mice.
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pubmed:affiliation |
Department of Microbiology and Immunology, University of North Carolina, Chapel Hill, NC, USA.
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pubmed:publicationType |
Journal Article
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