Linked Life Data

12829325

Source:http://linkedlifedata.com/resource/pubmed/id/12829325

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2003-6-27
pubmed:abstractText
The neurotrophic factor neurturin (NTN) is structurally related to the glial-derived neurotrophic factor (GDNF) and has been shown to prevent the degeneration of dopaminergic neurons both in vitro and in vivo. The preferred receptor for NTN is the GDNF family receptor alpha 2 (GFRalpha-2). To date, three protein-coding alternatively spliced GFRalpha-2 isoforms (GFRalpha-2a, GFRalpha-2b, GFRalpha-2c) have been identified in mammalian tissues. An accurate quantification of the expression levels is necessary when determining the contributions of these isoforms to NTN signaling in tissues. In this report, sequence independent real time RT-PCR is used to determine the expression levels of GFRalpha-2 isoforms at different developmental stages of the murine embryos, and in various adult tissues. In the adult murine brain, GFRalpha-2a was found to be the most abundant, GFRalpha-2c was slightly less and GFRalpha-2b was 10-fold lower. The testis did not appear to express significant levels of GFRalpha-2a, 2b or 2c, compared to the brain. A novel finding in this study is that in some tissues, including the adult brain, the expression levels of GFRalpha-2, as quantified by the amplification of the 3' sequences encoding the putative glycosyl-phosphatidylinositol anchor signal sequence, were significantly higher than the combined levels of GFRalpha-2a, GFRalpha-2b and GFRalpha-2c. This indicates the existence of yet to be identified forms of GFRalpha-2 in some tissues that may be of physiological significance.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0169-328X
pubmed:author
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
114
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
146-53
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:12829325-Aging, pubmed-meshheading:12829325-Alternative Splicing, pubmed-meshheading:12829325-Animals, pubmed-meshheading:12829325-Brain, pubmed-meshheading:12829325-Cell Differentiation, pubmed-meshheading:12829325-Fetus, pubmed-meshheading:12829325-Gene Expression Regulation, Developmental, pubmed-meshheading:12829325-Glial Cell Line-Derived Neurotrophic Factor Receptors, pubmed-meshheading:12829325-Mice, pubmed-meshheading:12829325-Nerve Growth Factors, pubmed-meshheading:12829325-Neurons, pubmed-meshheading:12829325-Neurturin, pubmed-meshheading:12829325-Polymerase Chain Reaction, pubmed-meshheading:12829325-Protein Isoforms, pubmed-meshheading:12829325-Proto-Oncogene Proteins, pubmed-meshheading:12829325-Proto-Oncogene Proteins c-ret, pubmed-meshheading:12829325-RNA, Messenger, pubmed-meshheading:12829325-Receptor Protein-Tyrosine Kinases, pubmed-meshheading:12829325-Viscera
pubmed:year
2003
pubmed:articleTitle
Real time PCR quantification of GFRalpha-2 alternatively spliced isoforms in murine brain and peripheral tissues.
pubmed:affiliation
Department of Biochemistry, National University of Singapore, Lower Kent Ridge Road, Singapore 119260, Singapore. bchtoohp@nus.edu.sg
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't