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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1993-2-11
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pubmed:abstractText |
The regulation of intercellular adhesion molecule-1 (ICAM-1) expression by renal tubular epithelial cells (TEC) has been studied in vitro. ICAM-1 expression in TEC is stimulated with phorbol 12-myristate 13-acetate (PMA), but not with forskolin, suggesting a role for protein kinase C (PKC) but not for protein kinase A (PKA). The tumor necrosis factor-alpha- (TNF-alpha) and interleukin-1 (IL-1)-stimulated ICAM-1 expression in TEC is blocked with the PKC/PKA inhibitor staurosporine, and also with the PKC-selective inhibitor calphostin C. The TNF-alpha-stimulated ICAM-1 expression is resistant however to downregulation of PKC with PMA. The TNF-alpha- and IL-1-stimulated ICAM-1 expression is also inhibited with the transcriptional inhibitor actinomycin D and with the protein synthesis inhibitor cycloheximide. Thus, ICAM-1 expression by TEC may involve a downregulation-resistant PKC which induces ICAM-1 expression at a transcriptional level.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1011-6524
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
15
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
302-6
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:1282723-Animals,
pubmed-meshheading:1282723-Antigens, CD,
pubmed-meshheading:1282723-Cell Adhesion Molecules,
pubmed-meshheading:1282723-Cell Line, Transformed,
pubmed-meshheading:1282723-Enzyme Activation,
pubmed-meshheading:1282723-Epithelium,
pubmed-meshheading:1282723-Gene Expression Regulation,
pubmed-meshheading:1282723-Intercellular Adhesion Molecule-1,
pubmed-meshheading:1282723-Interleukin-1,
pubmed-meshheading:1282723-Kidney Tubules,
pubmed-meshheading:1282723-Mice,
pubmed-meshheading:1282723-Protein Kinase C,
pubmed-meshheading:1282723-Tetradecanoylphorbol Acetate,
pubmed-meshheading:1282723-Transcription, Genetic,
pubmed-meshheading:1282723-Tumor Necrosis Factor-alpha
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pubmed:articleTitle |
Tumor necrosis factor-alpha- and interleukin-1-stimulated intercellular adhesion molecule-1 expression by murine renal tubular epithelial cells is transcriptionally regulated and involves protein kinase C.
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pubmed:affiliation |
Division of Nephrology, University of Alabama, Birmingham 35294.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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