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rdf:type |
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lifeskim:mentions |
umls-concept:C0033559,
umls-concept:C0079411,
umls-concept:C0086418,
umls-concept:C0205369,
umls-concept:C0334227,
umls-concept:C0346647,
umls-concept:C0441655,
umls-concept:C0442805,
umls-concept:C1565860,
umls-concept:C1705323,
umls-concept:C2611870
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pubmed:issue |
4
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pubmed:dateCreated |
2003-6-24
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pubmed:abstractText |
In some cancers cyclooxygenase (COX) inhibition appears to be anti-mitogenic and anti-angiogenic, but the actions of COX-derived prostaglandins in pancreatic cancer (PaCa) are unknown. In this study COX-2 was detected in three of six PaCa cell lines while COX-1 was identified in all cell lines. COX-2 expression correlated with basal and arachidonic acid (AA) stimulated PGE(2) production. PGE(2) production was inhibited by the COX-2 inhibitor nimesulide. In COX-2 expressing cells, exogenous AA and PGE(2) increased VEGF synthesis via the EP(2) receptor. Whereas PGE(2) stimulated intracellular cAMP formation in COX-2 positive and negative cells, 8-bromo cAMP stimulated VEGF production only in COX-2 expressing cells. Stimulating COX-2 expressing PaCa cell lines with AA enhanced migration of endothelial cells, an effect which was inhibited by a COX-2 inhibitor and EP(2) receptor antagonist. These data identify a subset of human PaCa cell lines that express functional COX-2 enzyme. PGE(2) generated by specific COX-2 activity increases VEGF secretion in human PaCa cells through an autocrine mechanism.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/8-Bromo Cyclic Adenosine...,
http://linkedlifedata.com/resource/pubmed/chemical/Arachidonic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase 2,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase 2 Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Dinoprostone,
http://linkedlifedata.com/resource/pubmed/chemical/Endothelial Growth Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Lymphokines,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/PTGS2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Prostaglandin-Endoperoxide Synthases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Sulfonamides,
http://linkedlifedata.com/resource/pubmed/chemical/Vascular Endothelial Growth Factor A,
http://linkedlifedata.com/resource/pubmed/chemical/Vascular Endothelial Growth Factors,
http://linkedlifedata.com/resource/pubmed/chemical/nimesulide
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
306
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
887-97
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12821125-8-Bromo Cyclic Adenosine Monophosphate,
pubmed-meshheading:12821125-Arachidonic Acid,
pubmed-meshheading:12821125-Blotting, Western,
pubmed-meshheading:12821125-Cell Movement,
pubmed-meshheading:12821125-Cells, Cultured,
pubmed-meshheading:12821125-Cyclic AMP,
pubmed-meshheading:12821125-Cyclooxygenase 2,
pubmed-meshheading:12821125-Cyclooxygenase 2 Inhibitors,
pubmed-meshheading:12821125-Cyclooxygenase Inhibitors,
pubmed-meshheading:12821125-Dinoprostone,
pubmed-meshheading:12821125-Dose-Response Relationship, Drug,
pubmed-meshheading:12821125-Endothelial Growth Factors,
pubmed-meshheading:12821125-Endothelium, Vascular,
pubmed-meshheading:12821125-Humans,
pubmed-meshheading:12821125-Intercellular Signaling Peptides and Proteins,
pubmed-meshheading:12821125-Isoenzymes,
pubmed-meshheading:12821125-Lymphokines,
pubmed-meshheading:12821125-Membrane Proteins,
pubmed-meshheading:12821125-Models, Biological,
pubmed-meshheading:12821125-Pancreatic Neoplasms,
pubmed-meshheading:12821125-Plasmids,
pubmed-meshheading:12821125-Prostaglandin-Endoperoxide Synthases,
pubmed-meshheading:12821125-RNA, Messenger,
pubmed-meshheading:12821125-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:12821125-Sulfonamides,
pubmed-meshheading:12821125-Time Factors,
pubmed-meshheading:12821125-Transfection,
pubmed-meshheading:12821125-Tumor Cells, Cultured,
pubmed-meshheading:12821125-Vascular Endothelial Growth Factor A,
pubmed-meshheading:12821125-Vascular Endothelial Growth Factors
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pubmed:year |
2003
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pubmed:articleTitle |
PGE(2) is generated by specific COX-2 activity and increases VEGF production in COX-2-expressing human pancreatic cancer cells.
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pubmed:affiliation |
Section of Gastrointestinal Surgery, David Geffen School of Medicine at UCLA, 10833 Le Conte Avenue, 72-231 CHS, Los Angeles, CA 90095-6904, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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