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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2003-6-23
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pubmed:abstractText |
ALG-2 was isolated in a screen for proteins involved in programmed cell death and is the first Ca(2+)-binding protein found to be directly involved in apoptosis. We have generated polyclonal antibodies that are suitable for detecting ALG-2 using different immunological methods. Three commercial antibodies against ALG-2 did neither detect mouse recombinant ALG-2 nor endogenous ALG-2 in Jurkat cell lysates, whereas our own affinity-purified antibody recognized recombinant as well as endogenous ALG-2. The specificity of the antibody was shown by preabsorbtion experiments and on ALG-2-deficient cells using Western blot analysis and immunohistochemistry. Western blot analysis of 15 different adult mouse tissues demonstrated that ALG-2 is ubiquitously expressed. We found that ALG-2 was more than threefold overexpressed in rat liver hepatoma compared to normal rat liver using Western blot analysis, a result confirmed by immunohistochemical analysis. Staining of four different lung cancer tissue microarrays including specimens of 263 patients showed that ALG-2 is mainly localized to epithelial cells and significantly up-regulated in small-cell lung cancers and in non-small-cell lung cancers. Our results lead to the conclusion that ALG-2 beside its known proapoptotic functions may be a player in survival pathways.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819013-10026166,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819013-10200558,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819013-10679202,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819013-10683420,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819013-10744743,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819013-10858458,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819013-10921882,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819013-11278427,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819013-11577478,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819013-11606059,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819013-11883939,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819013-11894095,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819013-12024023,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819013-12034747,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819013-12097266,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819013-12209154,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819013-12372597,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819013-12403687,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819013-12445460,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819013-12445461,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819013-4561027,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819013-8560270,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819013-8649366,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819013-9164928,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819013-9417038,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819013-9662379,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12819013-9880530
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0002-9440
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
163
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
81-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:12819013-Animals,
pubmed-meshheading:12819013-Antibodies,
pubmed-meshheading:12819013-Apoptosis,
pubmed-meshheading:12819013-Apoptosis Regulatory Proteins,
pubmed-meshheading:12819013-Calcium-Binding Proteins,
pubmed-meshheading:12819013-Carcinoma, Hepatocellular,
pubmed-meshheading:12819013-Humans,
pubmed-meshheading:12819013-Immunohistochemistry,
pubmed-meshheading:12819013-Liver Neoplasms,
pubmed-meshheading:12819013-Lung Neoplasms,
pubmed-meshheading:12819013-Mice,
pubmed-meshheading:12819013-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:12819013-Rats,
pubmed-meshheading:12819013-Tissue Distribution,
pubmed-meshheading:12819013-Tumor Cells, Cultured,
pubmed-meshheading:12819013-Up-Regulation
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pubmed:year |
2003
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pubmed:articleTitle |
Up-regulation of ALG-2 in hepatomas and lung cancer tissue.
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pubmed:affiliation |
Department of Molecular Cell Biology, University of Copenhagen, Copenhagen, and the Department of Pathology, University Hospital, Copenhagen, Denmark.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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