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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2003-6-23
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pubmed:abstractText |
Orexin-A has recently been identified as a new hypothalamic peptide working as a mediator in the regulation of feeding behavior and sleep control. To determine the role of orexin-A in peripheral metabolic processes, we examined direct effects of orexin-A on catecholamine synthesis and secretion in cultured bovine adrenal medullary cells. Incubation of cells with orexin-A (100 pM) for 20 min caused a small but significant increase in 14C-catecholamine synthesis from [14C]tyrosine, but not from L-3,4-dihydroxyphenyl[3-14C]alanine. Orexin-A (100 pM) potentiated the stimulatory effects of acetylcholine (0.3 mM) on 14C-catecholamine synthesis. Orexin-A significantly increased tyrosine hydroxylase activity, which was evident at 1 pM and maximal at 100 pM. 4 beta-Phorbol-12 beta-myristate-13 alpha-acetate, an activator of protein kinase C, did not enhance the stimulatory effects of orexin-A on tyrosine hydroxylase activity, while H-7 and staurosporine, inhibitors of protein kinase C, nullified the effects of orexin-A. Orexin-A had little effect on catecholamine secretion from the cells. Orexin receptor 1 (OX(1)R) but not orexin receptor 2 (OX(2)R) mRNA was detected in bovine adrenal medullary cells by reverse transcriptase-polymerase chain reaction. These findings suggest that orexin-A activates tyrosine hydroxylase and then stimulates catecholamine synthesis, probably via activation of the OX(1)R-protein kinase C pathway in adrenal medullary cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acetylcholine,
http://linkedlifedata.com/resource/pubmed/chemical/Carbon Radioisotopes,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Catecholamines,
http://linkedlifedata.com/resource/pubmed/chemical/Dihydroxyphenylalanine,
http://linkedlifedata.com/resource/pubmed/chemical/Intracellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Neuropeptides,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, G-Protein-Coupled,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Neuropeptide,
http://linkedlifedata.com/resource/pubmed/chemical/Tyrosine,
http://linkedlifedata.com/resource/pubmed/chemical/Tyrosine 3-Monooxygenase,
http://linkedlifedata.com/resource/pubmed/chemical/orexin receptors,
http://linkedlifedata.com/resource/pubmed/chemical/orexins
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0006-2952
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
66
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
141-7
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:12818374-Acetylcholine,
pubmed-meshheading:12818374-Adrenal Medulla,
pubmed-meshheading:12818374-Animals,
pubmed-meshheading:12818374-Carbon Radioisotopes,
pubmed-meshheading:12818374-Carrier Proteins,
pubmed-meshheading:12818374-Catecholamines,
pubmed-meshheading:12818374-Cattle,
pubmed-meshheading:12818374-Cells, Cultured,
pubmed-meshheading:12818374-Dihydroxyphenylalanine,
pubmed-meshheading:12818374-Enzyme Activation,
pubmed-meshheading:12818374-Intracellular Signaling Peptides and Proteins,
pubmed-meshheading:12818374-Neuropeptides,
pubmed-meshheading:12818374-Protein Kinase C,
pubmed-meshheading:12818374-Receptors, G-Protein-Coupled,
pubmed-meshheading:12818374-Receptors, Neuropeptide,
pubmed-meshheading:12818374-Time Factors,
pubmed-meshheading:12818374-Tyrosine,
pubmed-meshheading:12818374-Tyrosine 3-Monooxygenase
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pubmed:year |
2003
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pubmed:articleTitle |
Stimulation of catecholamine synthesis by orexin-A in bovine adrenal medullary cells through orexin receptor 1.
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pubmed:affiliation |
Department of Pediatrics, University of Occupational and Environmental Health, School of Medicine, 1-1, Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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