Source:http://linkedlifedata.com/resource/pubmed/id/12817010
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2003-6-20
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| pubmed:abstractText |
CD8(+) T cells are required for protective immunity against intracellular pathogens such as Listeria monocytogenes. In this study, we used class Ia MHC-deficient mice, which have a severe reduction in circulating CD8(+) T cells, to determine the protective capacity of class Ib MHC-restricted T cells during L. monocytogenes infection. The K(b-/-)D(b-/-) mutation was backcrossed onto a C.B10 (BALB/c congenic at H-2 locus with C57BL/10) background, because BALB/c mice are more susceptible to Listeria infection than other commonly studied mouse strains such as C57BL/6. C.B10 K(b-/-)D(b-/-) mice immunized with a sublethal dose of L. monocytogenes were fully protected against a subsequent lethal infection. Adoptive transfer of Listeria-immune splenocyte subsets into naive K(b-/-)D(b-/-) mice indicated that CD8(+) T cells were the major component of this protective immune response. A CD8(+) T cell line isolated from the spleen of a Listeria-infected class Ia MHC-deficient mouse was shown to specifically recognize Listeria-infected cells in vitro, as determined by IFN-gamma secretion and cytotoxicity assays. Adoptive transfer of this T cell line alone resulted in significant protection against L. monocytogenes challenge. These results suggest that even a limited number of class Ib MHC-restricted T cells are sufficient to generate the rapid recall response required for protection against secondary infection with L. monocytogenes.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
AIM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
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| pubmed:issn |
0022-1767
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
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| pubmed:volume |
171
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
291-8
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| pubmed:dateRevised |
2010-11-18
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| pubmed:meshHeading |
pubmed-meshheading:12817010-Adoptive Transfer,
pubmed-meshheading:12817010-Animals,
pubmed-meshheading:12817010-CD8-Positive T-Lymphocytes,
pubmed-meshheading:12817010-Cell Division,
pubmed-meshheading:12817010-Cell Line,
pubmed-meshheading:12817010-Cell Separation,
pubmed-meshheading:12817010-Crosses, Genetic,
pubmed-meshheading:12817010-Cytotoxicity, Immunologic,
pubmed-meshheading:12817010-Epitopes, T-Lymphocyte,
pubmed-meshheading:12817010-Female,
pubmed-meshheading:12817010-H-2 Antigens,
pubmed-meshheading:12817010-Immunity, Active,
pubmed-meshheading:12817010-Listeria monocytogenes,
pubmed-meshheading:12817010-Listeriosis,
pubmed-meshheading:12817010-Lymphocyte Activation,
pubmed-meshheading:12817010-Mice,
pubmed-meshheading:12817010-Mice, Congenic,
pubmed-meshheading:12817010-Mice, Inbred BALB C,
pubmed-meshheading:12817010-Mice, Inbred C57BL,
pubmed-meshheading:12817010-Mice, Knockout,
pubmed-meshheading:12817010-Mutation,
pubmed-meshheading:12817010-Spleen,
pubmed-meshheading:12817010-Tumor Cells, Cultured
|
| pubmed:year |
2003
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| pubmed:articleTitle |
Class Ia MHC-deficient BALB/c mice generate CD8+ T cell-mediated protective immunity against Listeria monocytogenes infection.
|
| pubmed:affiliation |
Department of Microbiology, Harvard Medical School, Boston, MA 02115, USA.
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| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|