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rdf:type |
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lifeskim:mentions |
umls-concept:C0004561,
umls-concept:C0010592,
umls-concept:C0028778,
umls-concept:C0040649,
umls-concept:C0086418,
umls-concept:C0205263,
umls-concept:C0332206,
umls-concept:C0729218,
umls-concept:C1456820,
umls-concept:C1537647,
umls-concept:C1637379
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pubmed:issue |
24
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pubmed:dateCreated |
1993-1-19
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pubmed:abstractText |
The human tumor necrosis factor alpha (TNF-alpha) gene encodes a cytokine whose activities have been implicated in many immunopathological processes, including the activation and differentiation of lymphocytes. Originally identified as a monocyte factor, our studies and those of others have demonstrated that B and T lymphocytes produce TNF-alpha when stimulated by a variety of inducers. We report here that TNF-alpha gene transcription is rapidly and highly induced in three independently derived human Burkitt lymphoma cell lines, as well as in freshly isolated human splenic B cells, activated by antibodies to surface immunoglobulin. This burst in TNF-alpha gene transcription is associated with an induction of TNF-alpha bioactivity in the culture supernatants from stimulated splenic B cells. Moreover, induction of TNF-alpha gene transcription by anti-immunoglobulin was blocked by the immunosuppressants cyclosporin A and FK506. These studies demonstrate that TNF-alpha production is an early event in B-cell activation and they establish the efficacy of using immunosuppressants as probes in dissecting transcriptional activation pathways in human B cells.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281550-1374612,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281550-1700753,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281550-1702384,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281550-1707162,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281550-1715516,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281550-1985116,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281550-2056282,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281550-2186752,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281550-2188669,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281550-2263603,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281550-2480331,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281550-2537976,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281550-2584936,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281550-2785134,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281550-2834453,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281550-2841782,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281550-2843816,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281550-2961351,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281550-3040886,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281550-3091258,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281550-317442,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281550-3263462,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281550-3489759,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281550-3497029,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281550-3871451,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281550-6319296,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281550-7014635,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281550-7464858
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
89
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
12198-201
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pubmed:dateRevised |
2010-9-7
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pubmed:meshHeading |
pubmed-meshheading:1281550-B-Lymphocytes,
pubmed-meshheading:1281550-Cyclosporine,
pubmed-meshheading:1281550-Gene Expression,
pubmed-meshheading:1281550-Humans,
pubmed-meshheading:1281550-Immunosuppressive Agents,
pubmed-meshheading:1281550-Lymphocyte Activation,
pubmed-meshheading:1281550-RNA, Messenger,
pubmed-meshheading:1281550-Receptor Aggregation,
pubmed-meshheading:1281550-Receptors, Antigen, B-Cell,
pubmed-meshheading:1281550-Tacrolimus,
pubmed-meshheading:1281550-Transcription, Genetic,
pubmed-meshheading:1281550-Tumor Cells, Cultured,
pubmed-meshheading:1281550-Tumor Necrosis Factor-alpha
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pubmed:year |
1992
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pubmed:articleTitle |
Transcription of the tumor necrosis factor alpha gene is rapidly induced by anti-immunoglobulin and blocked by cyclosporin A and FK506 in human B cells.
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pubmed:affiliation |
Division of Tumor Virology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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