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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1993-1-8
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pubmed:databankReference |
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pubmed:abstractText |
Signaling through the cell surface molecule, CD40, is known to play an important role in the proliferation and differentiation of B lymphocytes. Using the thymoma cell line EL4, we recently identified and cloned a cDNA encoding a murine ligand for the CD40 molecule (mCD40-L) and showed that it has biological activity in vitro. A cDNA encoding a human homologue of the mCD40-L was isolated using crosshybridization techniques from an activated peripheral blood T cell library. The predicted amino acid sequence indicates that this human ligand for CD40 (hCD40-L) is a 261 amino acid type II membrane protein that exhibits 78% amino acid identity with its murine counterpart. Northern blot and FACS analyses suggest that the hCD40-L is restricted in its expression to T lymphocytes, and that it is most abundant on the CD4+ T cell subpopulation. Cells transfected with hCD40-L caused the proliferation of human tonsil B cells in the absence of costimuli and, in the presence of interleukin 4, induced immunoglobulin E secretion from purified human B cells. A comparison of the efficacy of the hCD40-L and mCD40-L in these assays is presented.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281209-1310894,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281209-1348081,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281209-1370315,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281209-1378075,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281209-1379186,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281209-1701824,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281209-1706378,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281209-1706382,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281209-1716631,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281209-1833184,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281209-2148319,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281209-2160731,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281209-2463310,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281209-2475341,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281209-2476318,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281209-2594086,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281209-3487090,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281209-3500261,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1281209-6286831
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD40,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation...,
http://linkedlifedata.com/resource/pubmed/chemical/CD40 Ligand,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin E,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0022-1007
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
176
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1543-50
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pubmed:dateRevised |
2010-9-7
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pubmed:meshHeading |
pubmed-meshheading:1281209-Amino Acid Sequence,
pubmed-meshheading:1281209-Animals,
pubmed-meshheading:1281209-Antigens, CD,
pubmed-meshheading:1281209-Antigens, CD40,
pubmed-meshheading:1281209-Antigens, Differentiation, B-Lymphocyte,
pubmed-meshheading:1281209-B-Lymphocytes,
pubmed-meshheading:1281209-Base Sequence,
pubmed-meshheading:1281209-CD40 Ligand,
pubmed-meshheading:1281209-Cells, Cultured,
pubmed-meshheading:1281209-Cloning, Molecular,
pubmed-meshheading:1281209-Humans,
pubmed-meshheading:1281209-Immunoglobulin E,
pubmed-meshheading:1281209-Interleukin-4,
pubmed-meshheading:1281209-Kinetics,
pubmed-meshheading:1281209-Lymphocyte Activation,
pubmed-meshheading:1281209-Lymphoma,
pubmed-meshheading:1281209-Membrane Glycoproteins,
pubmed-meshheading:1281209-Mice,
pubmed-meshheading:1281209-Molecular Sequence Data,
pubmed-meshheading:1281209-Recombinant Proteins,
pubmed-meshheading:1281209-Sequence Homology, Amino Acid,
pubmed-meshheading:1281209-T-Lymphocytes,
pubmed-meshheading:1281209-Tumor Cells, Cultured
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pubmed:year |
1992
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pubmed:articleTitle |
Recombinant human CD40 ligand stimulates B cell proliferation and immunoglobulin E secretion.
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pubmed:affiliation |
Department of Molecular Biology, Immunex Research and Development Corporation, Seattle, Washington 98101.
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pubmed:publicationType |
Journal Article,
Comparative Study
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