Source:http://linkedlifedata.com/resource/pubmed/id/12797546
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
2003-6-11
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pubmed:abstractText |
Following lung injury, red blood cells (RBC) may interact with extracellular matrix (ECM). Fibroblasts, the resident cell in the ECM, have the capacity to produce and secrete a variety of mediators including interleukin-8 (IL-8). In the present study we hypothesized that RBC, or soluble factors released from them, may stimulate IL-8 production by fibroblasts. Fibroblasts were cultured in a three-dimensional collagen gel culture system in the presence or absence of RBC or conditioned medium from RBC (RBC-CM). IL-8 release from fibroblasts was significantly increased when cultured with RBC or RBC-CM and both tumor necrosis factor-alpha (TNF-alpha) and interleukin-1beta (IL-1beta) further stimulated this IL-8 secretion. The enhanced production of IL-8 within fibroblasts was accompanied by increased IL-8 mRNA expression. To evaluate whether RBC-fibroblast interaction may lead to recruitment of neutrophils, a functional migration assay was performed. RBC and RBC-CM, in the presence of IL-1beta and TNF-alpha, increased the transmigration of neutrophils. Our results indicate that RBC, when interacting with ECM, may participate in the recruitment of inflammatory cells by stimulating fibroblasts to secrete IL-8. This might be an important mechanism regulating tissue repair after injury.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Conditioned,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-8,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0360-3997
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
27
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
71-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12797546-Cells, Cultured,
pubmed-meshheading:12797546-Chemotaxis,
pubmed-meshheading:12797546-Culture Media, Conditioned,
pubmed-meshheading:12797546-Erythrocytes,
pubmed-meshheading:12797546-Fibroblasts,
pubmed-meshheading:12797546-Humans,
pubmed-meshheading:12797546-Interleukin-1,
pubmed-meshheading:12797546-Interleukin-8,
pubmed-meshheading:12797546-Lung,
pubmed-meshheading:12797546-Neutrophils,
pubmed-meshheading:12797546-Paracrine Communication,
pubmed-meshheading:12797546-RNA, Messenger,
pubmed-meshheading:12797546-Tumor Necrosis Factor-alpha
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pubmed:year |
2003
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pubmed:articleTitle |
Red blood cells stimulate human lung fibroblasts to secrete interleukin-8.
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pubmed:affiliation |
Department of Medicine, Division of Respiratory Medicine, Karolinska Hospital, Stockholm, Sweden. karin.fredriksson@ks.se
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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