Linked Life Data

12792819

Source:http://linkedlifedata.com/resource/pubmed/id/12792819

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2003-6-6
pubmed:abstractText
We attempted to raise the frequency of targeted gene repair attained by RNA/DNA chimeraplasty through activation of endogenous DNA recombination/repair system in cultured melan-c cells. For elevating the repair activity, either gamma-ray irradiation at 2 Gy or bleomycin addition at 5 nM to the culture medium was done. Subsequently, the tyrosinase-targeted chimeric RNA/DNA oligonucleotides were transfected. The frequency of targeted gene repair was assessed by three means, i.e., changes in cell color from transparent to dark black, restriction fragment band patterns, and DNA sequencing analysis. The results indicated that the gamma-ray irradiation did not improve the efficiency at all. In contrast, the low-dose bleomycin addition induced the frequency of targeted base conversion, compared with transfection with chimeric RDOs alone, although the frequency was still insufficient and varied from 0.1 to 10% of the total cultured cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1107-3756
pubmed:author
pubmed:issnType
Print
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
109-14
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Low-dose bleomycin induces targeted gene repair frequency in cultured melan-c cells using chimeric RNA/DNA oligonucleotide transfection.
pubmed:affiliation
Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan.
pubmed:publicationType
Journal Article