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rdf:type |
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lifeskim:mentions |
umls-concept:C0019682,
umls-concept:C0019691,
umls-concept:C0030657,
umls-concept:C0039194,
umls-concept:C0042776,
umls-concept:C0332281,
umls-concept:C0332307,
umls-concept:C0680063,
umls-concept:C1517945,
umls-concept:C1622204,
umls-concept:C1706853,
umls-concept:C1879748,
umls-concept:C2003941
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pubmed:issue |
12
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pubmed:dateCreated |
1992-12-15
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pubmed:abstractText |
Levels of human immunodeficiency virus (HIV) DNA, RNA, or p24 antigen and reverse transcriptase activity in T-cell cultures treated with 500 IU of recombinant alpha interferon (rIFN alpha) per ml were comparable to those in control cultures. Radioimmunoprecipitation analysis of proteins in lysates of IFN-treated T cells documented a marked accumulation of HIV proteins. Localization of gp120 by immunofluorescence showed a diffuse pattern in IFN-treated cells quite distinct from the ring pattern in untreated control cells. That large quantities of gp120 in aberrant cell compartments might affect HIV morphogenesis was confirmed in infectivity studies: virions from IFN-treated cells were 100- to 1,000-fold less infectious than an equal number of virions from control cells. Direct examination of IFN-treated and control HIV-infected cells by transmission electron microscopy showed little difference in the number or distribution of viral particles. However, quantitation of gp120 by immunogold particle analysis revealed a marked depletion of envelope glycoprotein in virions released from IFN-treated cells. This defect in gp120 assembly onto mature viral particles provides a molecular basis for this loss of infectivity.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279206-1573317,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279206-1686880,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279206-1688522,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279206-1707604,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279206-1710293,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279206-1711253,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279206-1715409,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279206-1738192,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279206-1906289,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279206-1971503,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279206-1976701,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279206-2213506,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279206-227998,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279206-2364019,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279206-2415251,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279206-2461053,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279206-2470148,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279206-2849111,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279206-3058820,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279206-3260631,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279206-3481357,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279206-6154948,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279206-6180108,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279206-6186764,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279206-6244697,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279206-6302994,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279206-6410234,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279206-7119475,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279206-79409,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279206-85689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1279206-88939
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/HIV Core Protein p24,
http://linkedlifedata.com/resource/pubmed/chemical/HIV Envelope Protein gp120,
http://linkedlifedata.com/resource/pubmed/chemical/HIV Reverse Transcriptase,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon Type I,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/RNA-Directed DNA Polymerase,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
66
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7543-8
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:1279206-Cells, Cultured,
pubmed-meshheading:1279206-DNA, Viral,
pubmed-meshheading:1279206-HIV Core Protein p24,
pubmed-meshheading:1279206-HIV Envelope Protein gp120,
pubmed-meshheading:1279206-HIV Reverse Transcriptase,
pubmed-meshheading:1279206-HIV-1,
pubmed-meshheading:1279206-Humans,
pubmed-meshheading:1279206-Interferon Type I,
pubmed-meshheading:1279206-RNA, Viral,
pubmed-meshheading:1279206-RNA-Directed DNA Polymerase,
pubmed-meshheading:1279206-Recombinant Proteins,
pubmed-meshheading:1279206-T-Lymphocytes,
pubmed-meshheading:1279206-Viral Proteins,
pubmed-meshheading:1279206-Virion
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pubmed:year |
1992
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pubmed:articleTitle |
Loss of infectivity by progeny virus from alpha interferon-treated human immunodeficiency virus type 1-infected T cells is associated with defective assembly of envelope gp120.
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pubmed:affiliation |
Department of Cellular Immunology, Walter Reed Army Institute of Research, Washington, D.C. 20307-5100.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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