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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2003-5-23
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pubmed:abstractText |
Typically, endothelial cells are difficult to transfect. In this study, we report that antennapedia peptide (AP) linked to L-4F, a water-soluble, amphipathic alpha helical peptide that avidly binds lipids (AP-4F) increases Lipofectamine 2000-mediated transfection of bovine coronary endothelial cell cultures. Transfection efficiency was monitored by flow cytometry and fluorescent microscopy. Lipofectamine 2000 transfection of endothelial cell cultures with green fluorescence protein (GFP)-DNA typically yields transfection efficiencies of 35.4+/-3.3% with low levels of cell death (8.1+/-1.0%). Pre-treatment of the Lipofectamine 2000-GFP-DNA complexes with AP-4F for 5 min increased transfection to 58.2+/-2.8% without increasing cell death. AP-4F increases Lipofectamine 2000-mediated transfection in a time-dependent fashion (within 10-20 min). Systematic studies reveal that the individual components of AP-4F, i.e., AP and L-4F alone, are ineffective in increasing Lipofectamine 2000-mediated transfection and that AP-4F must be directly associated with DNA liposomes prior to transfection for optimal uptake by endothelial cells. These observations demonstrate that AP-4F may be useful for increasing the transfection efficiency of endothelial cell cultures with standard commercially available reagents.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antennapedia Homeodomain Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Cation Exchange Resins,
http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Homeodomain Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/L-4F peptide,
http://linkedlifedata.com/resource/pubmed/chemical/Lipids,
http://linkedlifedata.com/resource/pubmed/chemical/Lipofectamine,
http://linkedlifedata.com/resource/pubmed/chemical/Liposomes,
http://linkedlifedata.com/resource/pubmed/chemical/Luminescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
6
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pubmed:volume |
305
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
605-10
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:12763037-Amino Acid Sequence,
pubmed-meshheading:12763037-Animals,
pubmed-meshheading:12763037-Antennapedia Homeodomain Protein,
pubmed-meshheading:12763037-Cation Exchange Resins,
pubmed-meshheading:12763037-Cattle,
pubmed-meshheading:12763037-Cells, Cultured,
pubmed-meshheading:12763037-Endothelium, Vascular,
pubmed-meshheading:12763037-Flow Cytometry,
pubmed-meshheading:12763037-Green Fluorescent Proteins,
pubmed-meshheading:12763037-Homeodomain Proteins,
pubmed-meshheading:12763037-Lipids,
pubmed-meshheading:12763037-Liposomes,
pubmed-meshheading:12763037-Luminescent Proteins,
pubmed-meshheading:12763037-Microscopy, Fluorescence,
pubmed-meshheading:12763037-Molecular Sequence Data,
pubmed-meshheading:12763037-Nuclear Proteins,
pubmed-meshheading:12763037-Peptides,
pubmed-meshheading:12763037-Transcription Factors,
pubmed-meshheading:12763037-Transfection
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pubmed:year |
2003
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pubmed:articleTitle |
AP-4F, antennapedia peptide linked to an amphipathic alpha helical peptide, increases the efficiency of Lipofectamine-mediated gene transfection in endothelial cells.
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pubmed:affiliation |
Division of Pediatric Surgery, Department of Surgery, Medical College of Wisconsin, Cardiovascular Center M4060, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't,
Evaluation Studies
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