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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
2003-5-2
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pubmed:abstractText |
In DNA-dependent RNA polymerases, reactions of RNA synthesis and degradation are performed by the same active center (in contrast to DNA polymerases in which they are separate). We propose a unified catalytic mechanism for multisubunit RNA polymerases based on the analysis of its 3'-5' exonuclease reaction in the context of crystal structure. The active center involves a symmetrical pair of Mg(2+) ions that switch roles in synthesis and degradation. One ion is retained permanently and the other is recruited ad hoc for each act of catalysis. The weakly bound Mg(2+) is stabilized in the active center in different modes depending on the type of reaction: during synthesis by the beta,gamma-phosphates of the incoming substrate; and during hydrolysis by the phosphates of a non-base-paired nucleoside triphosphate. The latter mode defines a transient, non-specific nucleoside triphosphate-binding site adjacent to the active center, which may serve as a gateway for polymerization of substrates.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/12727889-10499798,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12727889-10915625,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12727889-11313498,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12727889-11313499,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12727889-11511351,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12727889-12000971,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12727889-12408829,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12727889-12408830,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12727889-1371280,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12727889-14289336,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12727889-189795,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12727889-2068078,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12727889-226121,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12727889-6083781,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12727889-6986376,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12727889-7507490,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12727889-7538676,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12727889-7678416,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12727889-8058756,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12727889-8188709,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12727889-8286946,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12727889-8381534,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12727889-8431948,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12727889-8509421,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12727889-8658176,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12727889-8902816,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12727889-9050851,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12727889-9094712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12727889-9182561,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12727889-9192618,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12727889-9440683,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12727889-9514742,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12727889-9604937,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12727889-9857206
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0261-4189
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
22
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2234-44
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
2003
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pubmed:articleTitle |
Unified two-metal mechanism of RNA synthesis and degradation by RNA polymerase.
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pubmed:affiliation |
Public Health Research Institute, 225 Warren Street, Newark, NJ 07103, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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