Linked Life Data

12684836

Source:http://linkedlifedata.com/resource/pubmed/id/12684836

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2003-11-24
pubmed:abstractText
The aim of this study was to evaluate the effect of estrogen on matrix metalloproteinase (MMP)-1, MMP-3, MMP-13, and tissue inhibitor of metalloproteinase (TIMP)-1 in osteoarthritic chondrocytes. Chondrocytes from the knee cartilage of 25 postmenopausal osteoarthritic (OA) patients were cultured under various conditions: 0 pg/mL, 50 pg/mL, 500 pg/mL, and 5,000 pg/mL of 17beta-estradiol, with or without 10-1,000 pg/mL of either interleukin (IL)-1beta or tumor necrosis factor alpha (TNFalpha). MMP-1, MMP-3, MMP-13, and TIMP-1 in the conditioned media were analyzed with immunoblot or enzyme-linked immunosorbent assay (ELISA). Type II collagenolytic activity was measured by fluorogenic type II collagenolytic activity assay. Real-time reverse transcriptase polymerase chain reaction (RT-PCR) using SYBR Green I dye was performed for the quantification of mRNA. Without cytokine stimulation, the secretion of MMP-1 was significantly reduced by 50 pg/mL of 17beta-estradiol (in immunoblot by a median of 12.3%, P=0.007; in ELISA by a median of 18.4%, P=0.001), and 500 pg/mL (in immunoblot by a median of 23.1%, P=0.001; in ELISA by a median of 21.0%, P=0.001). Additionally, under 10 pg/mL TNFalpha, 17beta-estradiol also significantly suppressed the secretion of MMP-1 (in immunoblot by a median of 39.0%, P=0.016; in ELISA by a median of 38.4%, P=0.041). Estrogen did not exert any significant effect on MMP-3, MMP-13, or TIMP-1 expression. With IL-1beta or TNFalpha above 10 pg/mL stimulation, 17beta-estradiol demonstrated no effect on MMP-1, MMP-3, MMP-13, or TIMP-1 secretion. Type II collagenolytic activity in the 50 pg/mL estradiol group decreased by 9.6% (-51.5-5.5%, P>0.05). 17beta-estradiol showed a tendency to decrease in MMP-1 mRNA. Estrogen may improve the imbalance between the amounts of MMPs and TIMP in chondrocytes, and these results suggest that hormone replacement therapy may provide some chondroprotective effect.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0172-8172
pubmed:author
pubmed:issnType
Print
pubmed:volume
23
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
282-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12684836-Aged, pubmed-meshheading:12684836-Aged, 80 and over, pubmed-meshheading:12684836-Blotting, Western, pubmed-meshheading:12684836-Cartilage, Articular, pubmed-meshheading:12684836-Cells, Cultured, pubmed-meshheading:12684836-Chondrocytes, pubmed-meshheading:12684836-Culture Media, Conditioned, pubmed-meshheading:12684836-Dose-Response Relationship, Drug, pubmed-meshheading:12684836-Drug Combinations, pubmed-meshheading:12684836-Estradiol, pubmed-meshheading:12684836-Female, pubmed-meshheading:12684836-Humans, pubmed-meshheading:12684836-Interleukin-1, pubmed-meshheading:12684836-Matrix Metalloproteinases, pubmed-meshheading:12684836-Middle Aged, pubmed-meshheading:12684836-Osteoarthritis, Knee, pubmed-meshheading:12684836-Postmenopause, pubmed-meshheading:12684836-RNA, Messenger, pubmed-meshheading:12684836-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:12684836-Tissue Inhibitor of Metalloproteinase-1, pubmed-meshheading:12684836-Tumor Necrosis Factor-alpha
pubmed:year
2003
pubmed:articleTitle
Effect of estrogen on the expression of matrix metalloproteinase (MMP)-1, MMP-3, and MMP-13 and tissue inhibitor of metalloproternase-1 in osteoarthritis chondrocytes.
pubmed:affiliation
Department of Internal Medicine, Clinical Research Institute, Seoul National University College of Medicine, Seoul, Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't