Linked Life Data

12670346

Source:http://linkedlifedata.com/resource/pubmed/id/12670346

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2003-4-2
pubmed:abstractText
Natural killer T (NKT) cells are involved in innate immune defence and also in the regulation of adaptive immune responses. However, the development of NKT cells in vitro has not been fully characterized and culture conditions have not been fully optimized. In the present study, we found that an NKT cell fraction developed during the in vitro culture of cord blood (CB) CD34+ cells, and this was subsequently characterized both phenotypically and morphologically. CD34+ cells purified from 10 human CB were cultured in the presence of several cytokines and analysed by flow cytometry, light microscopy and electron microscopy. The NKT cell fraction, defined phenotypically (CD3+CD16+CD56+CD94+) as expressing the invariant T-cell receptor Valpha24 and Vbeta11, appeared in the CD56hi fractions. Intracytoplasmic staining demonstrated that interferon-gamma and interleukin 4 (IL-4) were detected in the CD56hi fractions. IL-15 was essential and, in combination with either flt3-ligand (FL) or stem cell factor (SCF), was sufficient to induce the development of NKT cells. The phenotype of the NKT cell fraction was CD45RO+CD45RA- and CD4+CD8alpha+. Morphologically, they were very large, with either round or oval nuclei, moderately condensed chromatins, voluminous weakly basophilic cytoplasm and various cytoplasmic granules such as dense core granules, multivesicular bodies, and intermediate form granules. When CD34+ cells purified from bone marrow (BM) were compared with those from CB, the latter were consistently more efficient at generating CD56hi NKT cell fractions. In conclusion, IL-15 in combination with FL and/or SCF can induce the differentiation of NKT cells from human CB CD34+ cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0007-1048
pubmed:author
pubmed:issnType
Print
pubmed:volume
121
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
148-56
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:12670346-Antigens, CD, pubmed-meshheading:12670346-Antigens, CD3, pubmed-meshheading:12670346-Antigens, CD56, pubmed-meshheading:12670346-CD4-Positive T-Lymphocytes, pubmed-meshheading:12670346-Cell Culture Techniques, pubmed-meshheading:12670346-Cell Differentiation, pubmed-meshheading:12670346-Cells, Cultured, pubmed-meshheading:12670346-Culture Media, pubmed-meshheading:12670346-Extracellular Matrix Proteins, pubmed-meshheading:12670346-Fetal Blood, pubmed-meshheading:12670346-Flow Cytometry, pubmed-meshheading:12670346-Humans, pubmed-meshheading:12670346-Immunophenotyping, pubmed-meshheading:12670346-Interleukin-15, pubmed-meshheading:12670346-Killer Cells, Natural, pubmed-meshheading:12670346-Lectins, C-Type, pubmed-meshheading:12670346-Microscopy, Electron, pubmed-meshheading:12670346-NK Cell Lectin-Like Receptor Subfamily D, pubmed-meshheading:12670346-Receptors, IgG, pubmed-meshheading:12670346-Stem Cell Factor
pubmed:year
2003
pubmed:articleTitle
In vitro differentiation of natural killer T cells from human cord blood CD34+ cells.
pubmed:affiliation
Department of Microbiology, College of Medicine, Ewha Womans University, Mok-6-Dong 911-1, Yangchon-Gu, Seoul 158-710, Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't