Source:http://linkedlifedata.com/resource/pubmed/id/12658443
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
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| pubmed:dateCreated |
2003-3-26
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| pubmed:abstractText |
Apoptosis of T cells contributes to the immune homeostasis in inflamed organs. A prominent T-cell infiltration is usually seen in human chronic active hepatitis, being associated with liver fibrosis. In order to demonstrate T-cell apoptosis in the hepatic fibrotic tissue, we induced T-cell infiltration in the fibrotic liver of the rat by injecting concanavalin A (Con A), a T-cell mitogen. Lymphocytes increased in number with a peak at 1 day, preferentially distributing in the fibrotic tissue rather than the parenchyma. They consisted of CD4-positive and CD8-positive cells, and gave the feature of lymphoblasts. Double staining for CD3 and TUNEL demonstrated that T cells underwent apoptosis. Apoptotic cells were more frequent in the fibrotic livers than the normal livers, and were spatially associated with alpha-smooth muscle actin-positive myofibroblast-like cells that possibly derived from hepatic stellate cells (HSCs) and portal fibroblasts through activation. In vitro experiments demonstrated that lymphocyte apoptosis was more frequently induced in the co-culture of Con A-activated splenic T cells/activated HSCs compared to that induced in activated T cells/quiescent HSCs or resting T cells/activated HSCs. The present results indicate that T cells which have extravasated and infiltrated the hepatic fibrotic tissue undergo apoptosis probably through an interaction with myofibroblast-like cells, suggesting the regulatory role of the latter cells in T-cell accumulation in the fibrotic liver.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Mar
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| pubmed:issn |
0302-766X
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
311
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
353-64
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| pubmed:meshHeading |
pubmed-meshheading:12658443-Actins,
pubmed-meshheading:12658443-Animals,
pubmed-meshheading:12658443-Antigens, CD3,
pubmed-meshheading:12658443-Apoptosis,
pubmed-meshheading:12658443-CD4-Positive T-Lymphocytes,
pubmed-meshheading:12658443-CD8-Positive T-Lymphocytes,
pubmed-meshheading:12658443-Cell Communication,
pubmed-meshheading:12658443-Cell Differentiation,
pubmed-meshheading:12658443-Chemotaxis, Leukocyte,
pubmed-meshheading:12658443-Concanavalin A,
pubmed-meshheading:12658443-Disease Models, Animal,
pubmed-meshheading:12658443-Fibroblasts,
pubmed-meshheading:12658443-Hepatitis,
pubmed-meshheading:12658443-In Situ Nick-End Labeling,
pubmed-meshheading:12658443-Liver Cirrhosis,
pubmed-meshheading:12658443-Male,
pubmed-meshheading:12658443-Microscopy, Electron,
pubmed-meshheading:12658443-Mitogens,
pubmed-meshheading:12658443-Rats,
pubmed-meshheading:12658443-Rats, Wistar,
pubmed-meshheading:12658443-T-Lymphocytes
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| pubmed:year |
2003
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| pubmed:articleTitle |
Apoptosis of T cells in the hepatic fibrotic tissue of the rat: a possible inducing role of hepatic myofibroblast-like cells.
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| pubmed:affiliation |
Department of Anatomy, Graduate School of Medicine, Osaka City University, 1-4-3 Asahimachi, Abeno-ku, 545-8585, Osaka, Japan. sawako@med.osaka-cu.ac.jp
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| pubmed:publicationType |
Journal Article
|